Contact us
닫기
Article Search
닫기

Microbiology and Biotechnology Letters

보문(Article)

View PDF

Food, Environment, and Other Topics in Biotechnology

Microbiol. Biotechnol. Lett. 2014; 42(4): 377-385

https://doi.org/10.4014/kjmb.1410.10001

Received: October 2, 2014; Accepted: December 4, 2014

고농도 염분폐수의 정화능이 우수한 기능성 미생물 커뮤니티의 군집 분석

Microbial Community Analysis in the Wastewater Treatment of Hypersaline-Wastewater

Jae-Won Lee 1, 2, Byung-Hyuk Kim 1, Yong-Seok Park 2, Young-Chae Song 1 and Sung-Cheol Koh 1*

1Department of Environmental Engineering, Korea Maritime and Ocean University, Busan 605-801, Republic of Korea, 2Q-BioTech Corporation, Ulasn 680-110, Republic of Korea

Abstract

Go to

In this study, a wastewater treatment system for hypersaline wastewater utilizing the Hypersaline Wastewater Treatment Community (HWTC) has been developed. The hypersaline wastewater treatment efficiency and microbial community of the HWTC were investigated. The average removal efficiencies of chemical oxygen demand were 84% in an HRT of 2.5 days. Microbial community analysis, by denaturing gradient gel electrophoresis (DGGE) of PCR-amplified 16S rRNA gene fragments and 16S rRNA gene clone library, revealed community diversity. The 16S rRNA gene analysis of dominant microbial bacteria in 4% hypersaline wastewater confirmed the presence of Halomonas sp. and Paenibacillus sp. Phylogenetic analysis suggested that the taxonomic affiliation of the dominant species in the HWTC was γ-proteobacteria and firmicutes. These results indicate the possibility that an appropriate hypersaline wastewater treatment system can be designed using acclimated sludge with a halophilic community.

Keywords: Wastewater treatment, Hypersalinity treatment, microbial community

References

Go to
  1. Abou-Elela SI, Kamel MM, Fawzy ME. 2010. Biological treatment of saline wastewater using a salt-tolerant microorganism. Desalination. 250: 1-5.
  2. APHA, AWWA, WEF. 2005. Standard methods for the examination of water and wastewater. APHA, Washington, DC, USA.
  3. Burnett WE. 1974. The effect of salinity variations on the activated sludge process. Water Sew. Works. 121: 37-38.
  4. Dastgheib SMM, Amoozegar MA, Khajeh K, Ventosa A. 2011. A halotolerant Alcanivorax sp. strain with potential application in saline soil remediation. Appl. Microbiol. Biotechnol. 90: 305312.
  5. de Albuquerque JP, Keim CN, Lins U. 2010. Comparative analysis of Beggiatoa from hypersaline and marine environments. Micron. 41: 507-517.
  6. Ishii K, Fukui M. 2001. Optimization of annealing temperature to reduce bias caused by a primer mismatch in multitemplate PCR. Appl. Environ. Microbiol. 67: 3753-3755.
  7. Jaspers E, Nauhaus K, Cypionka H, Overmann J. 2001. Multitude and temporal variability of ecological niches as indicated by the diversity of cultivated bacterioplankton. FEMS Microbiol. Ecol. 36: 153-164.
  8. Jin HJ, Tu R, Xu F, Chen SF. 2011. Identification of nitrogenfixing Paenibacillus from different plant rhizospheres and a novel nifH gene detected in the P. stellifer. Microbiology 80:117-124.
  9. Jung RK, Yang HC, Lee JJ, Kim YH. 2008. A novel halophilic Halomonas sp. having an excellent digestive effect of organic material, a soil conditioner for the reclaimed land using said strain and a method for plant growth-promoting thereof. Republic of Korea patent application.
  10. Kargi F, Dincer AR. 1998. Saline wastewater treatment by halophile-supplemented activated sludge culture in an aerated rotating biodisc contactor. Enzyme Microb. Tech. 22:427-433.
  11. Kargi F, Dincer AR. 1999. Salt inhibition of nitrification and denitrification in saline wastewater. Environ. Technol. 20:1147-1153.
  12. Kim B-H, Baek K-H, Cho D-H, Sung Y, Ahn C-Y, Oh H-M, et al. 2009. Analysis of microbial community during the anaerobic dechlorination of tetrachloroethylene (PCE) in stream of Gimpo and Inchon areas. Korean J. Microbiol. 45: 140-147.
  13. Kim JS, Pi YT. 2010. Oceanobacillus sp. strain having excellent salt-resistance and use thereof. Republic of Korea patent application.
  14. Kincannon DF, Gaudy AF. 1966. Some effect of high salt concentration on activated sludge. J. Water Poll. Cont. Fed. 38:1148-1158.
  15. Kincannon DF, Gaudy AF. 1968. Response of biological waste treatment system to changes in salt concentrations. Biotechnol. Bioeng. 10: 483-496.
  16. Kinner NE, Bishop PL, Asce M. 1962. Treatment of saline domestic wastewater using RBC's. J. Environ. Eng. ASCE. 108: 650-663.
  17. Kleinsteuber S, Riis V, Fetzer I, Harms H, Müller S. 2006. Population dynamics within a microbial consortium during growth on diesel fuel in saline environments. Appl. Environ. Microbiol. 72: 3531-3542.
  18. Lefebvre O, Vasudevan N, Thanasekaran K, Moletta R, Godon JJ. 2006. Microbial diversity in hypersaline wastewater:the example of tanneries. Extremophiles 10: 505-513.
  19. Liebgott P-P, Labat M, Casalot L, Amouric A, Lorquin J. 2007. Bioconversion of tyrosol into hydroxytyrosol and 3,4-dihydroxyphenylacetic acid under hypersaline conditions by the new Halomonas sp. strain HTB24. FEMS Microbiol. Lett. 276:23-33.
  20. Linarić M, Markić M, Sipos L. 2013. High salinity wastewater treatment. Water Sci. Technol. 68: 1400-1405.
  21. Ludzack FJ, Noran DK. 1965. Tolerance of high salinities by conventional wastewater treatment processes. J. Water Poll. Cont. Fed. 37: 1404-1413.
  22. Muyzer G. 1999. DGGE/TGGE a method for identifying genes from natural ecosystems. Curr. Opin. Microbiol. 2: 317-322.
  23. Muyzer G, Waal ECD, Uitterlinden AG. 1993. Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA. Appl. Environ. Microbiol. 59: 695700.
  24. Oren A, Gurevich P, Malkit A, Heins Y. 1992. Microbial degradation of pollutants at high salt concentrations. Biodegradation. 3: 387-398.
  25. Raud M, Tutt M, Jõgi E, Kikas T. 2012. BOD biosensors for pulp and paper industry wastewater analysis. Environ. Sci. Pollun. Res. 19: 3039-3045.
  26. Saratale RG, Saratale GD, Chang JS, Govindwar SP. 2010. Decolorization and biodegradation of reactive dyes and dye wastewater by a developed bacterial consortium. Biodegradation. 21: 999-1015.
  27. Sharghi EA, Bonakdarpour B, Roustazade P, Amoozegar MA, Rabbani AR. 2013. The biological treatment of high salinity synthetic oilfield produced water in a submerged membrane bioreactor using a halophilic bacterial consortium. J. Chem. Technol. Biotechnol. In press.
  28. Stewart MJ, Ludwig HF, Kearns WH. 1962. Effect of varying salinity on the extended aeration process. J. Water Poll. Cont. Fed. 34: 1161-1177.
  29. Surakasi VP, Antony CP, Sharma S, Patole MS, Shouche YS. 2010. Temporal bacterial diversity and detection of putative methanotrophs in surface mats of Lonar crater lake. J. Basic Microbiol. 50: 465-474.
  30. Tang J, Zheng A-P, Bromfield ESP, Zhu J, Li S-C, Wang S-Q, et al. 2011. 16S rRNA gene sequence analysis of halophilic and halotolerant bacteria isolated from a hypersaline pond in Sichuan, China. Ann. Microbiol. 61: 375-381.
  31. Tiquia SM, Davis D, Hadid H, Kasparian S, Ismail M, Ahly S, et al. 2007. Halophilic and halotolerant cacteria from river waters and shallow groundwater along the rouge river of Southeastern Michigan. Environ. Technol. 28: 297-307.
  32. Woolard CR, Irvine RL. 1994. Biological treatment of hypersaline wastewater by a biofilm of halophilic bacteria. Water Environ. Res. 66: 230-235.
  33. Woolard CR, Irvine RL. 1995. Treatment of hypersaline wastewater in the sequencing batch reactor. Water Res. 29:1159-1168.
  34. Yoshie S, Noda N, Miyano T, Tsuneda S, Hirata A, Inamori Y. 2001. Microbial community analysis in the denitrification process of saline-wastewater by denaturing gradient gel electrophoresis of PCR-amplified 16S rDNA and the cultivation method. J. Biosci. Bioeng. 92: 346-353.
  35. Yoshie S, Noda N, Tsuneda S, Hirata A, Inamori Y. 2004. Salinity decreases nitrite reductase gene diversity in denitrifying bacteria of wastewater treatment systems. Appl. Environ. Microbiol. 70: 3152-3517.

Article Tools

Starts of Metrics

Share this article on :

Related articles in MBL

Most Searched Keywords ?

What is Most Searched Keywords?

  • It is most registrated keyword in articles at this journal during for 2 years.