Contact us
닫기
Article Search
닫기

Microbiology and Biotechnology Letters

보문(Article)

View PDF

Food, Environment, and Other Topics in Biotechnology

Microbiol. Biotechnol. Lett. 2014; 42(3): 249-257

https://doi.org/10.4014/kjmb.1403.03001

Received: March 6, 2014; Accepted: May 29, 2014

백두구 추출물의 항산화 및 항비만 효과

Anti-Oxidative and Anti-Obesity Effects of Amomum Cardamomum L. Extract

Jung Ae Park 1, Kyong-Suk Jin 1, Ji Young Lee 1, Hyun Ju Kwon 1, 2 and Byung Woo Kim 1, 2*

1Blue-Bio Industry Regional Innovation Center, Dong-Eui University, Busan, 614-714, Korea, 2Department of Life Science and Biotechnology, College of Natural Science, Dong-Eui University, Busan, 614-714, Korea

Abstract

Go to

In this study, the anti-oxidative and anti-obesity activities of Amomum cardamomum L. methanol extract (ACME) were evaluated using DPPH radical scavenging activity assay, pancreatic lipase enzyme inhibition assay, and the cell culture model system. ACME exhibited DPPH radical scavenging activities dose-dependently, with IC50 of DPPH radical scavenging activities of ACME being 25.15 μg/ml. Furthermore, ACME effectively suppressed pancreatic lipase enzyme activity dose-dependently. ACME also significantly suppressed adipocyte differentiation, lipid accumulation, triglyceride (TG) contents, and triggered lipolysis activity on 3T3-L1 preadipocytes in a dose-dependent manner, without cytotoxicity. Their anti-obesity effect was modulated by the cytidine-cytidine-adenosine-adenosine-thymidine (CCAAT)/enhancer binding proteins α (C/EBPα), C/EBPβ and the peroxisome proliferator-activated receptor γ (PPARγ) gene and protein expressions. Taken together, these results provide an important new insight that A. cardamomum L. possesses anti-oxidative and anti-obesity activities such as pancreatic lipase inhibition, anti-adipogenic, and lipolysis effects. There is therefore potential for its use as a promising component in the field of nutraceuticals and the identification of the active compounds that confer the anti-oxidative and anti-obesity activities of ACME might be an appropriate next step.

Keywords: Amomum cardamomum L. methanol extract, anti-oxidative activity, anti-obesity activity, lipase inhibition activity, anti-adipogenic and lipolysis effects

References

Go to
  1. Acharya A, Das I, Singh S, Saha T. 2010. Chemopreventive properties of indole-3-carbinol, diindolylmethane and other constituents of cardamom against carcinogenesis. Recent Pat. Food Nutr. Agric. 2: 166-177.
    Pubmed CrossRef
  2. Attie AD, Scherer PE. 2009. Adipocyte metabolism and obesity. J. Lipid Res. 50: 395-399.
    Pubmed CrossRef
  3. Bae CR, Kwon DY, Cha YS. 2013. Anti-obesity effects of salted and unsalted doenjang supplementation in C57BL/6J mice fed with high fat diet. J. Korean Soc. Food Sci. Nutr. 42:1036-1042.
    CrossRef
  4. Cao Z, Umek RM, McKnight SL. 1991. Regulcated expresstion of three C/EBP isoforms during adipose conversion of 3T3-L1 cells. Genes Dev. 5: 1538-1552.
    Pubmed CrossRef
  5. Chen HC, Farese RV Jr. 2005. Inhibition of triglyceride synthesis as a treatment strategy for obesity: lessons from DGAT1-deficient mice. Arterioscler. Thromb. Vasc. Biol. 25:482-486.
    Pubmed CrossRef
  6. Chen Z, Torrens JI, Anand A, Spiegelman BM, Friedman JM. 2005. Krox20 stimulates adipogenesis via C/EBPbeta-dependent and –independent mechanisms. Cell Metab. 1: 93-106.
    Pubmed CrossRef
  7. Chua SC Jr. 1997. Monogenic models of obesity. Behav. Genet. 27: 277-284.
    Pubmed CrossRef
  8. Després JP, Lemieux I. 2006. Abdominal obesity and metabolic syndrome. Nature. 444: 881-887.
    Pubmed CrossRef
  9. Flier JS, Maratos Flier E. 1998. Obesity and the hypothalamus:novel peptides for new pathways. Cell. 92: 437-440.
    CrossRef
  10. Gesta S, Tseng YH, Kahn CR. 2007. Developmental origin of fat: Tracking obesity to its source. Cell. 131: 242-256.
    Pubmed CrossRef
  11. Gregoire FM, Smas CM, Sul HS. 1998. Understanding adipocyte differentiation. Physiol. Rev. 78: 783-809.
    Pubmed
  12. Gupta R, Rathi P, Gupta N, Bradoo S. 2003. Lipase assays for conventional and molecular screening: an overview. Biotechnol. Appl. Biochem. 37: 63-71.
    Pubmed CrossRef
  13. Heck AM, Yanovski JA, Calis KA. 2000. Orlistat, a new lipase inhibitor for the management of obesity. Pharmacotherapy. 20: 270-279.
    Pubmed CrossRef
  14. Hotamisligil GS. 2006. Inflammation and metabolic disorders. Nature. 444: 860-867.
    Pubmed CrossRef
  15. Kedare SB, Singh RP. 2011. Genesis and development of DPPH method of antioxidant assay. J. Food Sci. Technol. 48:412-422.
    Pubmed CrossRef
  16. Kim SH, Kim JY, Ryu KA, Sohn CM. 2007. Evaluation of the dietary diversity and nutrient intakes in obese adults. Korean J. Community Nutr. 12: 583-591.
  17. Kim EJ, Kim GY, Kim YM, Choi KH, Jang SJ. 2009. Anti-obesity effect of mulberry leaves extraction in obese rats high-fat diet. Korean J. Oriental Physiol. Pathol. 23: 831-836.
  18. Lee DH, Kang SS, Chang IM, Mar W. 1997. Detection of antiinflammatory agents from natural products as inhibitors of cyclooxygenase I and II. Nat. Prod. Sci. 3: 19-28.
  19. Liu F, Kim J, Li Y, Liu X, Li J, Chen X. 2001. An extract of Lagerstroemia speciosa L. has insulin-like uptake-stimulatory and adipocyte differentiation-inhibitory activities in 3T3-L1 cells. J. Nutr. 131: 2242-2247.
    Pubmed
  20. Majdalawieh AF, Carr RI. 2010. In vitro investigation of the potential immunomodulatory and anti-cancer activities of black pepper (Piper nigrum) and cardamom (Elettaria cardamomum). J. Med. Food. 13: 371-381.
    Pubmed CrossRef
  21. Morrison RF, Farmer SR. 2000. Hormonal signaling and transcriptional control of adipocyte differentiation. J. Nutr. 130:3116-3121.
  22. Ntambi JM, Kim YC. 2000. Adipocyte differentiation and gene expression. J. Nutr. 130: 3122-3126.
  23. Park JA, Park C, Han MH, Kim BW, Chung YH, Choi YH. 2011. Inhibition of adipocyte differentiation and adipogenesis by aged black garlic extracts in 3T3-L1 preadipocytes. J. Life Sci. 21: 720-728.
    CrossRef
  24. Rosen ED, Macdougald OA. 2006. Adipocyte differentiation from the inside out. Nat. Rev. Mol. Cell Biol. 7: 885-896.
    Pubmed CrossRef
  25. Suzuki R, Tanaka M, Takanashi M, Hussain A, Yuan B, Toyoda H, et al. 2011. Anthocyanidins-enriched bilberry extracts inhibit 3T3-L1 adipocyte differentiation via the insulin pathway. Nutr. Metab. (Lond). 8: 8-14.
    Pubmed CrossRef
  26. Vogel C, Marcotte EM. 2012. Insights into the regulation of protein abundance from proteomic and transcriptomic analyses. Nat. Rev. Genet. 13: 227-232.
    Pubmed
  27. Wu Z, Bucher NL, Farmer SR. 1996. Indeuction of peroxisome proliferator-activated receptor gamma during the conversion of 3T3 fibroblasts into adipocytes is mediated by C/EBP beta, C/EBP delta, and glucocorticoids. Mol. Cell Biol. 16: 4128-4136.
    Pubmed
  28. Wu Z, Xie Y, Bucher NL, Farmer SR. 1995. Conditional etopic expression of C/EBP beta in NIH-3T3 cells induces PPAR gamma and stimulates adipogenesis. Genes Dev. 9: 23502363.
    CrossRef
  29. Yeh WC, Cao Z, Classon M, McKnight SL. 1995. Cascade regulation of terminal adipocyte differentiation by three members of the C/EBP family of leucine zipper proteins. Genes Dev. 9: 168-181.
    Pubmed CrossRef
  30. Zhang JW, Klemm DJ, Vinson C, Lane MD. 2004. Role of CREB in transcriptional regulation of CCAAT/enhancer-binding protein beta gene during adipogenesis. J. Biol. Chem. 279: 4471-4478.
    Pubmed CrossRef

Article Tools

Starts of Metrics

Share this article on :

Related articles in MBL

Most Searched Keywords ?

What is Most Searched Keywords?

  • It is most registrated keyword in articles at this journal during for 2 years.