Contact us
닫기
Article Search
닫기

Microbiology and Biotechnology Letters

단보(Note)

View PDF

Fermentation and Bioprocess Engineering

Microbiol. Biotechnol. Lett. 2014; 42(2): 202-206

https://doi.org/10.4014/kjmb.1403.03005

Received: March 11, 2014; Accepted: April 1, 2014

효모를 이용한 에탄올 생산을 위한 감껍질 전처리조건의 최적화

Optimization of Pretreatment of Persimmon Peel for Ethanol Production by Yeast Fermentation

Jong-Sub Lee 1, Eun-Hee Park 1, Se-Young Kwun 1, Soo-Hwan Yeo 2 and Myoung-Dong Kim 1*

1Department of Food Science and Biotechnology, Kangwon National University, Chuncheon 200-701, Republic of Korea, 2Fermentation and Food Processing Division, Department of Agrofood Resources, NAAS, RDA, Suwon 411-853, Republic of Korea

Abstract

Go to

A response surface method based on a central composite design experiment was used to determine the optimum conditions for pretreatment of persimmon peel. It was mathematically predicted that the maximum amount of reducing sugars would be obtained at an H2SO4 concentration of 1.77% (w/v) and a heat treatment time of 26.4 min. A reducing sugar concentration of 63.23 g/l was obtained under the optimum pretreatment conditions determined by RSM. Under anaerobic growth conditions, Saccharomyces cerevisiae NK28 produced 15.52 g/l of ethanol with a yield of 0.34 g ethanol/g glucose from pretreated persimmon peel, which corresponded to 14% and 26% enhancements in ethanol productivity and ethanol yield, respectively, compared with those obtained in aerobic growth conditions. This study suggests that persimmon peel might be a useful substrate for bioethanol production by yeast fermentation.

Keywords: Saccharomyces cerevisiae, persimmon peel, fermentation, pretreatment, response surface method

References

Go to
  1. Cho WS, Chung YC, Kim BK, Suh SJ, Koh WS, Choe SH. 2007. Cellulosic ethanol as renewable alternative fuel. J. Plant Biotechnol. 34: 111-118.
    CrossRef
  2. Choi SJ, Lee SM, Lee JH. 2012. Production of bio-ethanol from red algae by acid hydrolysis and enzyme treatment. Appl. Chem. Eng. 23: 279-283.
  3. Eyini M, Rajapandy V, Parani K, Lee MW. 2004. Effect of different pretreatment methods on the bioconversion of rice bran into ethanol. Korean Soc. Mycol. 32: 170-172.
  4. Goshima T, Tsuji M, Inoue H, Yano S, Hoshino T, Matsushika A. 2013. Bioethanol production from lignocellulosic biomass by a novel Kluyveromyces marxianus strain. Biosci. Biotechnol. Biochem. 77: 1505-1510.
    CrossRef
  5. Grahovac JA, Dodić JM, Dodić SN, Popov SD, Jokić AI. 2011. Optimization of bioethanol production from intermediates of sugar beet processing by response surface methodology. Biomass Bioenerg. 35: 4290-4296.
    CrossRef
  6. Heer D, Sauer U. 2008. Identification of furfural as a key toxin in lignocellulosic hydrolysates and evolution of a tolerant yeast strain. Microb. Biotechnol. 1: 497-506.
    CrossRef
  7. Jeong TS, Oh KK. 2009. Behaviors of glucose decomposition during dilute-acid hyrdolysis of lignocellulosic biomass. Korean Soc. Biotechnol. Bioeng. J. 24: 267-272.
  8. Kim HY, Lee JW, Jeffries TW, Choi IG. 2011. Evaluation of oxalic acid pretreatment condition using response surface method for producing bio-ethanol from Yellow poplar (Liriodendron tulipifera) by simulataneous saccharification and fermentation. Mokchae Konghak 39: 75-85.
  9. Kim KS, Kim JS. 2010. Optimization of ammonia percolation process for ethanol production from miscanthus sinensis. Korean Chem. Eng. Res. 48: 704-711.
  10. Kim SK, Lee GD, Chung SK. 2003. Monitoring on fermentation of persimmon vinegar from persimmon peel. Korean J. Food Sci. Technol. 35: 642-647.
  11. Klinke HB, Thomsen AB, Ahring BK. 2004. Inhibition of ethanolproducing yeast and bacteria by degradation products produced during pre-treatment of biomass. Appl. Microbiol. Biotechnol. 66: 10-26.
    CrossRef
  12. Ko JJ, Yun S, Kang SW, Kim SK. 2008. A review on thermochemical pretreatment in lignocellulosic bioethanol production. Korea Organic Res. Recy. Assoc. 16: 79-88.
  13. Krahulec S, Petschacher B, Wallner M, Longus K, Klimacek M, Nidetzky B. 2010. Fermentation of mixed glucose-xylose substrates by engineered strains of Saccharomyces cerevisiae:role of the coenzyme specificity of xylose reductase, and effect of glucose on xylose utilization. Microb. Cell Fact. 9: 16.
    CrossRef
  14. Lee GD, Jeong YJ. 1998. Optimization on organoleptic properties of Kochujang with addition of persimmon fruits. J. Korean Soc. Food Sci. Nutr. 27: 1132-1136.
  15. Lee JS, Park EH, Kim JW, Yeo SH, Kim MD. 2011. Growth and fermentation characteristics of Saccharomyces cerevisiae NK28 isolated from kiwi fruit. J. Microbiol. Biotechnol. 39:33-39.
  16. Lee JW, Kim HY, Jeffries TW, Choi IG. 2010. Bioethanol production using by-product of VPP(value prior to pulping). Mokchae Konghak 38: 561-567.
  17. Lee SB, Lee JD. 2010. Effect of pretreatment process on cellulosic ethanol production using waste papers. J. Korean Solid Wastes Eng. Soc. 27: 553-557.
  18. Lee SM, Choi IS, Kim SK, Lee JH. 2009. Production of bioethanol from brown algae by enzymic hydrolysis. Korean Soc. Biotechnol. Bioeng. J. 24: 483-488.
  19. Lee SM, Lee JH. 2010. Influence of acid and salt content on the ethanol production from Laminaria japonica. Appl. Chem. Eng. 21: 154-161.
  20. Lim WS, Lee JW. 2012. Enzymatic hydrolysis condition of pretreated corncob by oxalic acid to improve ethanol production. J. Korea Wood Sci. Technol. 40: 294-301.
    CrossRef
  21. Lu X, Zhang Y, Angelidaki I. 2009. Optimization of H2SO4-catalyzed hydrothermal pretreatment of rapeseed straw for bioconversion to ethanol: focusing on pretreatment at high solids content. Bioresour. Technol. 100: 3048-3053.
    CrossRef
  22. McLaren JS. 2005. Crop biotechnology provides an opportunity to develop a sustainable future. Trends Biotechnol. 23:339-342.
    CrossRef
  23. Miller GL. 1959. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal. Chem. 31: 426-428.
    CrossRef
  24. Oh KK, Hong SI, Lee YY. 1998. Optimization of ammonia recycled percolation process for lignocellulosic biomass pretreatment. Korean J. Chem. Eng. 36: 784-791.
  25. Palmqvist E, Grage H, Meinander NQ, Hahn-Hägerdal B. 1999. Main and interaction effects of acetic acid, furfural, and p-hydroxybenzoic acid on growth and ethanol productivity of yeasts. Biotechnol. Bioeng. 63: 46-55.
    CrossRef
  26. Piccolo C, Bezzo F. 2009. A techno-economic comparison between two technologies for bioethanol production from lignocellulose. Biomass Bioenerg. 33: 478-491.
    CrossRef
  27. Saha BC, Iten LB, Cotta MA, Wu YV. 2005. Dilute acid pretreatment, enzymatic saccharification, and fermentation of rice hulls to ethanol. Biotechnol. Prog. 21: 816-822.
    CrossRef
  28. Sassner P, Galbe M, Zacchi G. 2008. Techno-economic evaluation of bioethanol production from three different lignocellulosic materials. Biomass Bioenerg. 32: 422-430.
    CrossRef
  29. Taherzadeh MJ, Eklund R, Gustafsson L, Niklasson C, Lidèn G. 1997. Characterization and fermentation of dilute-acid hydrolyzates from wood. Ind. Eng. Chem. Res. 36: 46594665.
    CrossRef
  30. Tran AV, Chabmers PR. 1986. Lignin and extractives derived inhibitors in the 2, 3-butanediol fermentation of mannose-rich prehydrolysates. Appl. Microbiol. Biotechnol. 23: 191-197.
    CrossRef
  31. Vilaplana M, García AB, Caminal G, Guillén F, Sarrà M. 2012. Optimisation of the operational conditions of trichloroethylene degradation using trametes versicolor under quinone redox cycling conditions using central composite design methodology. Biodegradation 23: 333-341.
    CrossRef
  32. Won KY, Oh KK. 2009. Optimization the xylose fractionation conditions of pepper stem with dilute sulfuric acid. Korean Soc. Biotechnol. Bioeng. J. 24: 361-366.

Article Tools

Starts of Metrics

Share this article on :

Related articles in MBL

Most Searched Keywords ?

What is Most Searched Keywords?

  • It is most registrated keyword in articles at this journal during for 2 years.