Contact us
닫기
Article Search
닫기

Microbiology and Biotechnology Letters

보문(Article)

View PDF

Fermentation and Bioprocess Engineering

Microbiol. Biotechnol. Lett. 2013; 41(4): 407-415

https://doi.org/10.4014/kjmb.1311.11001

Received: November 1, 2013; Accepted: December 9, 2013

황칠나무 잎 추출물의 세포 항산화 활성과 미백활성 측정

Cellular Antioxidant Activity and Whitening Effects of Dendropanax morbifera Leaf Extracts

Su Ah Park 1, Jun Park 2, Chan Il Park 2, Young Jong Jie 2, Yun Chan Hwang 2, Yong Hyun Kim 2, So Ha Jeon 1, Hye Mi Lee 1, Ji Hoon Ha 1, Kyeong Jin Kim 1 and Soo Nam Park 1*

1Department of Fine Chemistry, Nanobiocosmetic laboratory, and Cosmetic R&D center, Seoul National University of Science and Technology, Seoul 139-743, Korea, 2Seoul Science High School, Seoul 110-521, Korea

Abstract

Go to

In this study, we investigated the antioxidant activities on HaCaT and the whitening effects on B16F1 melanoma cells of Dendropanax morbifera leaf extract. In an antioxidative activity assay using HaCaT cells, the ethyl acetate (50 μg/ml) and aglycone fractions (25 μg/ml) of the D. morbifera leaf extract didn't exhibit any characteristics of cytotoxicity. When HaCaT cells were exposed to a single large dose (800 mJ/cm2) of UVB, the extracts protected the cells against UVB radiation. When HaCaT cells were treated with 10 mM H2O2 and 4 μM rose bengal, the ethyl acetate (6.25~50 μg/ml) and aglycone (6.25~25 μg/ml) fractions protected the cells against oxidative damage in a concentration dependent manner. When the whitening effects of D. morbifera leaf extract were tested in melanoma B16/F1 cells treated with the a-melanocyte stimulating hormone (α-MSH), the extracts inhibited α-MSH-stimulated intra/extracellular melanogenesis in a concentration dependent manner. The inhibitory effects of the ethyl acetate and aglycone fractions of D. morbifera leaf extract were 21% and 44% at 25 μg/ml, respectively. Both are more effective than arbutin (15% at 25 μg/ml) which is known as a whitening agent. These results indicate that fractions of the D. morbifera leaf can function as cell protectants and natural antioxidants in biological systems, particularly skins exposed to UV radiation by quenching and/or scavenging 1O2 and other ROS, and protecting cells against ROS. In addition, fractions of the D. morbifera leaf can be applied to new whitening cosmetics because of their inhibitory effects on α-MSH stimulated melanogenesis in B16F1 melanoma cells.

Keywords: Dendropanax morbifera, antioxidant activity, HaCaT, B16F1 melanoma, a-MSH, melanogenesis

References

Go to
  1. Ahn YJ, Won BR, Kang MK, Kim JH, Park SN. 2009. Antioxidant activity and component analysis of fermented Lavandula angustifolia extracts. J. Soc. Cosmet. Scientists Korea 35:125-134.
  2. Ando H, Niki Y, Ito M, Akiyama K, Matsui MS, Yarosh DB, et al. 2012. Melanosomes are transferred from melanocytes to keratinocytes through the processes of packaging, release, uptake, and dispersion. J. Invest. Dermatol. 132: 1222-1229.
    Pubmed CrossRef
  3. Han SB, Gu HA, Kim SJ, Kim HJ, Kwon SS, Kim HS, et al. 2013. Comparative study on antioxidative activity of Glycyrrhiza uralensis and Glycyrrhiza glabra extracts by country of origin. J. Soc. Cosmet. Scientists Korea 39: 1-8.
  4. Hosoi J, Abe E, Suda T, Kuroki T. 1985. Regulation of melanin synthesis of B16 mouse melanoma cell by 1α, 25-dihydroxyvitamin D3 and retinoic acid. Cancer Res. 45: 14741478.
  5. Hyuna TK, Kim M, Lee H, Kim Y, Kim E, Kim J-S. 2013. Evaluation of anti-oxidant and anti-cancer properties of Dendropanax morbifera Léveille. Food Chemistry 141: 1947-1955.
    Pubmed CrossRef
  6. Iwata M, Corn T, Iwata S, Everett MA, Fuller BB. 1990. The relationship between tyrosinase activity and skin color in human foreskins. J. Invest. Dermatol. 95: 9-15.
    Pubmed CrossRef
  7. Jeong BS, Jo JS, Pyo BS, Hwang B. 1995. Studies on the distribution of Dendropanax morbifera and component analysis of the golden lacquer. Korean J. Biotechnol. Bioeng. 10:393-400.
  8. Jo NR, Park MA, Chae KY, Park SA, Jeon SH, Ha JH, et al. 2012. Cellular protective and antioxidative acivities of Parthenocissus tricuspidata stem extracts. J. Soc. Cosmet. Scientists Korea 38: 225-236.
  9. Kameyama K, Takemura T, Hamada Y, Sakai C, Kondoh S, Nishi-yama S. 1993. Pigment production in murine melanoma cells is regulated by tyrosinase, tyrosinase-related protein 1 (TRP), dopachrome tautomerase (TRP 2) and a melanogenic inhibitor. J. Invest. Dermatol. 100: 126-132.
    Pubmed CrossRef
  10. Kim AR, Park SA, Ha JH, Park SN. 2013. Antioxidative, and inhibitory activities on melanogenesis of Vitex negundo L. leaf extract. Korean J. Microbiol. Biotechnol. 41: 135-144.
    CrossRef
  11. Kim HR, Chung HJ. 2000. Chemical characteristics of the leaves and the seeds of Korean Dendropanax (Dendropanax morvifera Lev.). J. Korean Soc. Agric. Chem. Biotechnol. 43:63-66.
  12. Packer L. 1994. Ultraviolet radiation (UVA, UVB) and skin antioxidants, In C. A. Rice-Evans and R. H. Burdon (ed), Free radical damage and its control, 239: Elsevier Science, Amsterdam.
    CrossRef
  13. Park SN. 2003. Antioxidative properties of baicalein, component from Scutellaria baicalensis Georgi and its application to cosmetics (I). J. Korean Ind. Eng. Chem. 14: 657-665.
  14. Park SN. 1989. Dissertation, Seoul National Univ., Seoul, Korea.
  15. Park SN. 1997. Skin aging and antioxidant. J. Soc. Cosmet. Scientists Korea 23: 75-132.
  16. Pillai S, Oresajo C, Hayward J. 2005. Ultraviolet radication and skin aging: roles of reactive oxygen species, inflammation and protease activation, and strategies for prevention of inflammation-induced matrix degradation-a review. Int. J. Cosmet. Science 27: 17-34.
    Pubmed CrossRef
  17. Rhie GE, Shin MH, Seo JY, Choi WW, Cho KH, Kim KH, et al. 2011. Aging- and photoaging-dependent changes of enzymic and nonenzymic antioxidants in the epidermis and dermis of human skin in vivo. J. Invest. Dermatol. 117: 1212-1217.
    Pubmed CrossRef
  18. Scharffetter-Kochanek K. 1997. Photoaging of the connective tissues of skin: Its prevention and therapy, antioxidants in disease mechanism and therapy. Adv. Pharmacol. 38: 639-655.
    Pubmed
  19. Seiberg M. 2001. Keratinocyte-melanocyte interation during melanosome transfer. Pigment. Cell Res. 14: 236-242.
    Pubmed CrossRef
  20. Seo EJ, Hong ES, Choi MH, Kim KS, Lee SJ. 2010. antioxidant and skin whitening effects of Rhamnus yoshinoi extracts. Korean J. Food Sci. Technol. 42: 750-754.
  21. Seo H, Seo GY, Ko SZ, Park YH. 2011. Inhibitory effects of ethanol extracts from Polygoni multiflori radix and Cynanchi wilfordii radix on melanogenesis in melanoma cells. J. Korean Soc. Food Sci. Nutr. 40: 1086-1091.
    CrossRef
  22. Steinbeck MJ, Khan AU, Karmovsky MJ. 1992. Intracellular singlet oxygen generation by phagocytosing neutrophils in response to particles coated with a chemical trap. J. Biol. Chem. 267: 13425-13433.
    Pubmed
  23. Weixiong L, Helene ZH. 1997. Induced melanin reduces mutations and cell killing in mouse melanoma. Phytochem Phytobiol. 65: 480-485.
    CrossRef
  24. Won DH, Han SB, Hwang JP, Kim SJ, Park JN, Park SN. 2012. Antioxidative effect and tyrosinase inhibitory activity of Lindera obtusiloba Blume extracts. J. Soc. Cosmet. Scientists Korea 38: 297-304.
  25. Yaar M, Gilchrest BA. 2007. Photoageing: mechanism, prevention and therapy. Br. J. Dermatol. 157: 874-887.
    Pubmed CrossRef
  26. Yamakoshi J, Otsuka F, Sano A, Tokutake S, Saito M, Kikuchi M, et al. 2003. Lightening effect on ultraviolet-induced pigmentation of guinea pig skin by oral administration of a proanthocyanidinrich extract from grape seeds. Pig. Cell Res. 16:629-638.
    CrossRef

Article Tools

Starts of Metrics

Share this article on :

Related articles in MBL

Most Searched Keywords ?

What is Most Searched Keywords?

  • It is most registrated keyword in articles at this journal during for 2 years.