Contact us
닫기
Article Search
닫기

Microbiology and Biotechnology Letters

보문(Article)

View PDF

Fermentation and Bioprocess Engineering

Microbiol. Biotechnol. Lett. 2013; 41(2): 190-197

https://doi.org/10.4014/kjmb.1302.02004

Received: February 14, 2013; Accepted: April 29, 2013

전통 장류로부터 Exopolysaccharide 생성 유산균의 분리 및 특성

Isolation and Characterization of Exopolysaccharide Producing Lactic Acid Bacteria from Korean Soy Sauce and Soybean Paste

Hye Ju Yun 1, 2, You Jung Lee 1, Soo-Hwan Yeo 1, Hye Young Park 1, Heui-Dong Park 2 and Seong Yeol Baek 1*

1Fermented Food Division, Department of Agro-food Resource, NAAS, RDA, Suwon 441-853, Korea, 2School of Food Science & Biotechnology, Kyungpook National University, Daegu 702-701, Korea

Abstract

Go to

Three slime-forming lactic acid bacteria were isolated from traditional Korean fermented soy sauce and soybean paste and shown to produce exopolysaccharides (EPS) in sucrose media. By isolating the strains, examining their morphological characteristics and determining their 16S rDNA sequences, N58-5 and K6-7 were identified as Leuconostoc mesenteroides and N45- 10 as Leuconostoc citreum. The acid and bile tolerances of these three strains were investigated. Amongst the three lactic acid bacteria, Leuc. citreum N45-10 exhibited the highest viability (105-106 CFU/ml) in 0.05 M sodium phosphate buffer (pH 0.3) for 2 h, in artificial gastric juice for 2 h and in 0.3%, 0.5% oxgall for 24h. Leuc. mesenteroides K6-7, N58-5 and Leuc. citreum N45- 10 were grown in sucrose liquid medium and 8.16 g/L, 3.65 g/L, 16.17 g/L of EPS was collected, respectively. The hydrolyzed EPS was analyzed by HPLC in order to determine the sugar composition of EPS. Leuc. mesenteroides K6-7 and N58-5 showed two peaks indicating glucose and fructose, thus they were determined to be hetero-type polysaccharides. Leuc. citreum N45-10 showed only the glucose polymer, indicating it to be a homo-type polysaccharide. In addition, all three lactic acid bacterial hemolysis did not demonstrate a clear zone in blood agar in the area surrounding a lactic acid bacteria colony.

Keywords: Lactic acid bacteria, exopolysaccharide, soy sauce, Leuconostoc sp.

References

Go to
  1. Aguirre, M. and M. D. Collins. 1993. Lactic acid bacteria and human clinical infection. J. Appl. Bacteriol. 75: 95-107.
    Pubmed CrossRef
  2. Ahn, Y. S., Y. S. Kim, and D. H. Shin. 2006. Isolation, identification and fermentation characteristics of Bacillus sp. with high protease activity from traditional cheonggukjang. Korean J. Food Sci. Technol. 38: 82-87.
  3. Bernard, F. G., Z. Alexandre, M. Robert, and M. Catherine. 2004. Production and characterization of bioactive peptides from soy hydrolysate and soy-fermented food. Food Res. Int. 37: 123-131.
    CrossRef
  4. Brook, I. 1996. Isolation of non-sporing anaerobic rods from infections in children. J. Med. Microbiol. 45: 21-6.
    Pubmed CrossRef
  5. Chou, L. S. and B. Weimer. 1999. Isolation and chraracterization of acid- and bile-tolerant isolates from strains of Lactobacillus acidophilus. J. Dairy Sci. 82: 23-31.
    CrossRef
  6. Duboc, P. and B. Mollet. 2001. Applications of expolysaccharides in the dairy industry. Int. Dairy J. 11: 759-768.
    CrossRef
  7. Dufresne, R., J. Thibault, A. Leduy, and R. Lencki. 1990. The effects of pressure on the growth of Aureobasidium pullulans and the synthesis of pullulan. Appl. Microbiol. Biotech. 32:526-532.
    CrossRef
  8. Fu, J. F. and Y. H. Tseng. 1990. Construction of lactose-utilizing Xanthomonas campestris and production of xanthan gum from whey. Appl. Environ. Microbiol. 56: 919-923.
    Pubmed KoreaMed
  9. Gasser, F. 1994. Safety of lactic acid bacteria and their occurrence in human clinical infections. Bull. Inst. Pasteur. 92: 4567.
  10. Hood, S. K. and E. A. Zittola. 1998. Effect of low pH on the ability of Lactobacillus acidophilus to survive and adhere to human intestinal cells. J. Food Sci. 53: 1514-1516.
    CrossRef
  11. Isenberg, H. D. 1992. Gelatin liquefaction test. Clincal Microbiology Procedures Handbook. Vol. 1, pp. 1.19.42-1.19.43. American Socity of microbiology, Washington D.D., USA.
  12. Jeanes, A., D. A. Wilham, H. M. Tsuchiya, and W. C. Haynes. 1957. Properties of dextrans isolated from whole cultures at various stages of incubation. Arch. Biochem. Biophys. 71:293-302.
    CrossRef
  13. Kang, H. J., S. C. Baick, and J. H. Yu. 2005. Studies on the properties of the stirred yogurt manufactured by exopolysaccharide producing lactic acid bacteria. Korean J. Food Sci. Ani. Resour. 25: 84-91.
  14. Kim, D. J. and S. Y. Lee. 2001. Isolation of exopolysaccharide producing Enterobacter sp. and physicochemical properties of the polysaccharide produced by this strain. Korean J. Biotechnol. Bioeng. 16: 370-375.
  15. Kim, H. J. and H. C. Chang. 2006. Isolation and characterization of exopolysaccharide producing lactic acid bacteria from kimchi. Korean J. Microbiol. Biotechnol. 34: 196-203.
  16. Kim, Y. M., U. K. Park, J. S. Mok, and D. S. Chang. 1995. Physiological characteristics of Listeria monocytogenes YM-7. J. Korean Fish Soc. 28: 443-450.
  17. Kimmel, S. A., R. F. Roberts, and G. R. Ziegler. 1998. Optimization of exopolysaccharide production by Lactobacillus delbrueckii subsp. bulgaricus RR grown in a semidefined medium. Appl. Environ. Microbiol. 64: 659-664.
    Pubmed KoreaMed
  18. Kobayashi, Y., K. Tohyama, and T. Terashima. 1974. Biological characteristics of Lactobacillus. II. Tolerance of the multiple antibiotic resistance strain, L. casei PSR3002, to artificial digestive fluids. Jpn. J. Microbiol. 29: 691-697.
  19. Lee, J. J., Y. M. Lee, H. C. Chang, and M. Y. Lee. 2007. Acute toxicity of Leuconostoc kimchii GJ2, an exopolysaccharideproducing lactic acid bacteria isolated from kimchi, in mice. J. Life Sci. 17: 561-567.
    CrossRef
  20. Lee, Y. and H. C. Chang. 2008. Isolation and characterization of kimchi lactic acid bacteria showing anti-Helicobacter pylori activity. Korean J. Microbiol. Biotechnol. 36: 106-114.
  21. Lee, Y. N., M. J. Sin, and B. N. Kim. 1991. A study on the present state of traditional food. Korean J. Dietary. Culture. 6:71-81.
  22. Oh, J. H., B. J. Lee, H. R. Paik, S. C. Jung, K. S. Baik, and S. K. Choi. 2009. Isolation of bacteria from chunggukjang prepared by rice straw and identification of protease secreted. J. Life Sci. 19: 397-402.
    CrossRef
  23. Paik, H. D., M. Y. Jung, H. Y. Jung, W. S. Kim, and K. T. Kim. 2002. Characterization of Bacillus polyfermenticus SCD for oral bacteriotherapy of gastrointestinal disorders. Korean J. Food Sci. Technol. 34: 73-78.
  24. Park, J. G., S. Y. Yun, S. Oh, J. G. Shin, and Y. J. Baek. 2003. Probiotic characteristics of Lactobacillus acidophilus KY1909 isolated from Korean breast-fed infant. Korean J. Food Sci.Technol. 35: 1244-1247.
  25. Patricia, R. M., J. Hugenholtz, and P. Zoon. 2002. An overview of the functionality of exopolysaccharides produced by lactic acid bacteria. Int. Dairy J. 12: 163-171.
    CrossRef
  26. Saitou, N. and M. Nei. 1987. The neighbor joining-methods: a new method for reconstructig phylogenetic trees. Mol. Biol. Evol. 4: 406-425.
    Pubmed
  27. Shin, H. J. 2004. Studies on the probiotic characteristics of L. paracasei LA44 isolated from Korean feces. Department of Food Biotechnology Graduate School Sungkyunkwan University.
  28. Shin, M. S., J. J. Lee, S. H. Na, H. S. Bae, C. S. Huh, and Y. J. Baek. 1998. Characteristics of Bifidobacterium spp. Isolated from korean faces for probiotics. Korean J. Dairy Sci. 20: 273282.
  29. Smitinont, T., C. Tansakul, S. Tanasupawat, S. Keeratipibul, L. Navarini, M. Bosco, and P. Cescutti. 1999. Exopolysaccharideproducing lactid acid bacteria streins from traditional thai fermented foods: isolation, identification and exopolysaccharide characterization. Int. J. Food Microbiol. 51: 105-111.
    CrossRef
  30. Sutherland, I. W. 1972. Bacterial exopolysaccharides. Adv. Microbiol. Physiol. 8: 143-213.
    CrossRef
  31. Sutherland, I. W. 1998. Novel and established applications of microbial polysaccharides. Trends in Biotechnol. 16: 41-46.
    CrossRef
  32. Sutherland, I. W. 1996. Extracellular polysaccharides. pp. 613-657, In H. J. Rehm and G. Reed (eds.), Biotechnology: A Multi-Volume Comprehensive Treatise. 2nd, completely revised ed., vol. 6, VCH, Verlagsgesellschaft, Weinheim, Germany.
  33. Van den Berg, D. J. C., G. W. Robijn, A. C. Janssen, M. L. F. Giuseppin, R. Vreeker, J. P. Kamerling, J. F. G. Vliegenthart, A. M. Ledeboer, and C. T. Verrips. 1995. Production of a novel extracellular polysaccharide by Lactobacillus sake 0-1 and characterization of the polysaccharide. Appl. Environ. Microbiol. 61: 2840-2844.
    Pubmed KoreaMed
  34. Yang, Z., E. Huttunen, M. Staaf, G. Widmalm, and H. Tenhu. 1999. Separation, purification and characterization of extracellular polysaccharides produced by slime-forming Lactococcus lactis ssp. cremoris strains. Int. Dairy J. 9: 631-638.
    CrossRef

Article Tools

Starts of Metrics

Share this article on :

Related articles in MBL

Most Searched Keywords ?

What is Most Searched Keywords?

  • It is most registrated keyword in articles at this journal during for 2 years.