Contact us
닫기
Article Search
닫기

Microbiology and Biotechnology Letters

보문(Article)

View PDF

Food, Environment, and Other Topics in Biotechnology

Microbiol. Biotechnol. Lett. 2013; 41(1): 96-104

https://doi.org/10.4014/kjmb.1208.08012

Received: August 30, 2012; Accepted: November 13, 2012

식품에서 분리한 황색포도상구균의 항생제 내성 특징 및 균막 형성

Antibiotic Resistance and Bacterial Biofilm Formation by Staphylococcus aureus Strains Isolated from Various Foods

Joo-Young Lee 1, Hae-Jin Wang 1, Dong-Bin Shin 1 and Yong-Sun Cho 1*

Food Analysis Center, Korea Food Research Institute, Gyeonggi-do 463-746, Korea

Abstract

Go to

Staphylococcus aureus is a major human pathogen that produces a wide array of toxins, leading to a number of adverse symptoms. We examined 275 strains of Staphylococcus aureus isolated from various foods between 2006 and 2008 for antimicrobial susceptibility. At least 259 (94.2%) of the tested strains showed antibiotic resistant properties, and 106 (40.7%) of them showed multiple antibiotic resistance. Eleven of the tested strains were resistant to oxacillin and mec A-positive. Moreover, oxacillin-resistant strains were significantly more likely to be multi-drug resistant (p < 0.01). Of the 275 isolates tested, 24.4% were noted as being positive for slime production and 30.5% were positive for biofilm assay. Antibiotic resistance was not associated with a significantly higher prevalence of biofilm formation. Twenty strains were classified using the DiversiLab system. Most of the strains could be classified into 2 clusters and 4 unique types. All 10 mec A-positive strains (cluster I) were grouped together into the same sub-cluster. Cluster II (6 strains) was not found to be resistant to oxacillin in this study. Although the prevalence of methicillin-resistant S. aureus in food is currently low, the risk of its transmission through the food chain cannot be disregarded.

Keywords: Staphylococcus aureus, antibiotic resistance, biofilm, MRSA

References

Go to
  1. Chery, K. S., J. I. Pounder, S. R. Page, B. J. Schaecher, and G. L. Woods. 2005. Clinical Evaluation of the DiversiLab microbial typing system using repetitive-sequence-based PCR for characterization of Staphylococcus aureus strains. J. Clin. Microbiol. 43: 1187-1192.
    Pubmed KoreaMed CrossRef
  2. Chmielewski, R. A. N. and J. F. Frank. 2003. Biofilm formation and control in food processing facilities. Compr. Rev. Food Sci. Food Saf. 2: 22-32.
    CrossRef
  3. Cho, K. J., S. Jin, J. H. Cui, T. R. Yoon, and P. Y. Ryu. 2008. Effects of biofilm formation on the antimicrobial susceptibility of Staphylococcus aureus. J. Bacteriol. Virol. 38: 197-205.
    CrossRef
  4. Freeman, D. J., F. R. Falkiner, and C. T. Keane. 1989. New method for detecting slime production by coagulase negative staphylococci. J. Clin. Pathol. 42: 872-874.
    Pubmed KoreaMed CrossRef
  5. Hwang, I. G., H. S. Kwak, and S. H. Yoon. 2010. Methicillinresistant Staphylococcus aureus (MRSA) as a foodborne biological hazard. J. Food Hyg. Safety 50: 26-36.
  6. Johannes, K. M., A. H. Matthiab, R. Hologer, and M. Dietrich. 2002. Evaluation of different detection methods of biofilm formation in Staphylococcus aureus. Med. Microbiol. Immunol. 191: 101-106.
    Pubmed CrossRef
  7. KFDA. 2009. Repot of 2009 Estabililshment of a risk profile for methicillin-resistant Staphylococcus aureus (MRSA). Korea Food and Drug Administration, Cheongwon, Korea.
  8. KFDA. 2010. Statistical yearbook. Food - borne disease outbreaks, Korea Food and Drug Administration, Cheongwon, Korea.
  9. Kim, J. S., H. S. Kim, W. Song, H. C. Cho, K. M Lee, and E. C. Kim. 2004. Antimicrobial ressistat profiles of Staphylococcus aureus isolated in 13 Korea hospitals. Korean J. Lab. Med. 24: 223-229.
  10. Kumar, C. G. and S. K. Anand. 1998. Significance of microbial biofilms in food industry. Int. J. Food Microbiol. 42: 9-27.
    CrossRef
  11. Kwon, A.S., G. C. Park,, S. Y. Ryu, D. H. Lim, D. Y. Lim, C. H. Choi, Y. K. Park, and Y. Lim. 2008. Higher biofilm formation in multidrug-resistant clinical isolates of Staphylococcus aureus. Int. J. Antimicrob. Agents 32: 68-72.
    Pubmed CrossRef
  12. Kwon, Y. I., T. W. Kim, H. Y. Kim, Y. H. Chang, H. S. Kwak, and G. J. Woo. 2007. Monitoring of methicillin resistant Staphylococcus aureus from medical environment in Korea. Korean J. Microbiol. Biotechnol. 35: 158-162.
  13. Lee, W. C., T. Sakai, M. J. Lee, M. Hamakawa, S. M. Lee, and I. M. Lee. 1996. An epidemiological study of food poisoning in Korea and Japan. Int. J. Food Microbiol. 29: 141-148 .
    CrossRef
  14. Lim, S. K., H. M. Nam, H. J. Park, H. S. Lee, M. J. Choi, S. C. Jung, J. Y. Lee, Y. C. Kim, S. W. Song, and S. H. Wee. 2010. Prevalence and characterization of methicillin -resistant Staphylococcus aureus in raw meat in Korea. J. Microbiol. Biotechnol. 20: 775-778.
    Pubmed KoreaMed CrossRef
  15. Marco, L., B. Cinzia, G. B. Maria, F. Maria, Z. Jessica, and F. Roberta. 2002. Evaluation of the VITEK 2 system for identification and antimicrobial susceptibility testing of medically relevant gram-positive cocci. J. Clin. Microbiol. 40: 1681-1686.
    Pubmed CrossRef
  16. Park J. I. and N. L. Lee. 1998. Comparison of susceptibility test and meca detection for determination of methicillin resistance in Staphylococcus epidermidis. Korean J. Clin. Pathol. 18: 391-395.
  17. Pérez-Roth, E., F. C. Martýìn, J. Villar, and S. M. Álvarez. 2001. Multiplex PCR for simultaneous identification of Staphylococcus aureus and detection of methicillin and mupirocin resistance. J. Clin. Microbiol. 39: 4037-4041.
  18. Philip, S. S. and J. W. Costerton. 2001. Antibioric resistance of bacteria in biofilms. J. Lancet. 358: 135-138.
  19. Rhee, C. H. and G. J. Woo. 2010. Emergence and characterization of foodborne methicillin-resistant Staphylococcus aureus in Korea. J. Food Prot. 73: 2285-2290.
  20. Ross, T. L., W. G. Merz, M. Farkosh, and K. C. Carroll. 2005. Comparison of an automated repetitive sequence-based pcr microbial typing system to pulsed-field gel electrophoresis for analysis of outbreaks of methicillin-resistant Staphylococcus aureus. J. Clin. Microbiol. 43: 5642-5647.
  21. Shutt, C.K., J. I. Pounder, S. R. Page, B. J. Schaecher, and G. L. Woods. 2005. Clinical evaluation of the diversilab microbial typing system using repetitive-sequence-based PCR for characterization of Staphylococcus aureus strains. J. Clin. Microbiol. 43: 1187-1192.
  22. Timothy, F. J., M. E. Kellum, S. S. Porter, M. Bell, and W. Schaffner. 2002. An Outbreak of community-acquired foodborne illness caused by methicillin-resistant Staphylococcus aureus. J. Emerg. Infect. Dis. 8: 82-84.
  23. Vasudevan, P., K. M. N. Manoj, T. Annamalai, and K. S. Venkitanarayanan. 2003. Phenotypic and genotypic characterization of bovine mastitis isolates of Staphylococcus aureus for biofilm formation. Vet. Microbiol. 92: 179-185.
  24. Wei, H. L. and C. S. Chiou. 2002. Molecular subtyping of Staphylococcus aureus from an outbreak associated with a food handler. Epidemiol. Infect. 128: 15-20.
  25. Witta, R., V. Kanhaib, and W. B. Leeuwena. 2009. Comparison of the DiversiLabTM system, pulsed-field gel electrophoresis and multi-locus sequence typing for the characterization of epidemic reference MRSA strains. J. Microbiol. Methods 77:130-133.

Article Tools

Starts of Metrics

Share this article on :

Related articles in MBL

Most Searched Keywords ?

What is Most Searched Keywords?

  • It is most registrated keyword in articles at this journal during for 2 years.