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Microbiology and Biotechnology Letters

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Food, Environment, and Other Topics in Biotechnology

Microbiol. Biotechnol. Lett. 2012; 40(3): 250-260

https://doi.org/10.4014/kjmb.1207.07014

Received: July 18, 2012; Accepted: August 13, 2012

사자발쑥 추출물의 항산화 및 항균 활성

Antioxidative and Antibacterial Activities of Artemisia princeps Pampanini Extracts

Hyun Gab Yang 1, Hye Jin Kim 1, Hae Soo Kim 1 and Soo Nam Park 1*

Department of Fine Chemistry, Nanobiocosmetic Laboratory, and Cosmetic R&D Center, Seoul National University of Science and Technology, Seoul 139-743, Korea

Abstract

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In the present study, the antioxidative and antibacterial activities of Artemisia princeps Pampanini (A. princeps Pamp.) extract were investigated. The ethyl acetate fraction of A. princeps Pamp. showed the most prominent free radical (1,1-diphenyl-2-picrylhydrazyl, DPPH) scavenging activity (FSC50 = 12.27 μg/mL). Reactive oxygen species (ROS) scavenging activities (OSC50) of A. princeps Pamp. extract on Fe3+-EDTA/ H2O2 systems were investigated using a luminol-dependent chemiluminescence assay. The ethyl acetate fraction of the extract (OSC50 = 0.33 μg/mL) had a 5 times greater ROS scavenging activity than L-ascorbic acid (1.50 μg/mL), known as a water soluble antioxidant. The cellular protective effects of fractions of A. princeps Pamp. on the rose-bengal sensitized photohemolysis of human erythrocytes were examined. The aglycone fraction of extracts suppressed photohemolysis in a concentration dependent manner. The inhibitory effects of A. princeps Pamp. extract on tyrosinase were investigated to assess their whitening efficiency. The ethyl acetate fraction demonstrated a 7 times higher tyrosinase inhibitory effect (IC50 = 29.20 μg/mL) than albutin, known as a whitening agent. The antibacterial activity of ethyl acetate fractions against various normal skin flora were measured. The results showed that the antibacterial activity of the fraction was the highest on Staphylococcus aureus, Bacillus subtilis, and Propionibacterium acnes. Antioxidant substances were isolated and purified from the ethyl acetate fractions. Eupatilin and jaceosidin were identified. These results indicate that the extract/fractions of A. princeps Pamp. can function as antioxidant and/or antibacterial agents for the skin.

Keywords: Artemisia princeps Pampanini, reactive oxygen species, antioxidant, flavonoids, cosmetics

References

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