Contact us
닫기
Article Search
닫기

Microbiology and Biotechnology Letters

보문(Article)

View PDF

Molecular and Cell biology, and Microbial Isolation

Microbiol. Biotechnol. Lett. 2012; 40(3): 190-196

https://doi.org/10.4014/kjmb.1207.07001

Received: July 3, 2012; Accepted: September 6, 2012

미생물제제시용 고추경작지로부터 식물생장홀몬과 항진균물질을 동시에 생산하는 식물생장촉진근권세균의 선발 및 동정

Selection and Identification of Phytohormones and Antifungal Substances Simultaneously Producing Plant Growth Promoting Rhizobacteria from Microbial Agent Treated Red-pepper Fields

Byung-Kwon Jung 1, Jong-Hui Lim 1, Chang-Hwan An 1, Yo-Hwan Kim 1 and Sang-Dal Kim 1*

Department of Applied Microbiology and Biotechnology School of Biotechnology, Yeungnam University, Gyeongsan 712-749, Korea

Abstract

Go to

In this study, a total of more than 1,000 bacteria, including 739 species of aerobic bacteria, 80 species of urease producing bacteria and 303 species of photosynthetic bacteria, were isolated from red-pepper field soils located in the Gyeongsan Province of the Republic of Korea. Amongst these, 158 species of aerobic bacteria, 70 species of urease producing bacteria and 228 species of photosynthetic bacteria were found to be auxin producing soil bacteria through quantification analysis with the Salkowski test. The latter groupings were then tested for antifungal activities to β-Glucanase and siderophore using CMC congo red agar and CAS blue agar media. In addition, the selected strains were examined for antifungal activity against various phytopathogenic fungi on PDN agar media. Six strains; BCB14, BCB17, C10, HA46, HA143, and HJ5, were noted for their ability to both produce auxin and act as antifungal substances. 16S rDNA sequence comparison analyses of these six strains identified them as Bacillus subtilis BCB14, B. methylotrophicus BCB17, B. methylotrophicus C10, B. sonorensis HA46, B. subtilis HA143, and B. safensis HJ5.

Keywords: microbial agent, phytohormone, PGPR, antifungal activity

References

Go to
  1. Amara, M. A. T. and M. S. A. Dahdoh. 1997. Effect of inoculation with plant growth promoting rhizobacteria (PGPR) on yield and uptake of nutrients by wheat grown on sandy soil. Egyptian J. Soil Science 37: 467-484.
  2. Ates, S., S. Ozenir, and M. Gökdere. 2006. Effect of silicone oil on gibberellic acid production by Gibberella fujikuroi and Aspergillus niger. Appl. Biochem. Microbiol. 42: 500-501.
    CrossRef
  3. Barthe, P., V. Pujade-Renaud, F. Breton, D. Gargani, R. Thai, and C. Roumestand. 2007. Structural analysis of cassiicolin, a host-selective protein toxin from Corynespora cassiicola. J. Mol. Biol. 367: 89-101.
    Pubmed CrossRef
  4. Bomke, C. and B. Tudzynski. 2009. Diversity, regulation, and evolution of the gibberellin biosynthetic pathway in fungi compared to plants and bacteria. Phytochemistry 70:1876-1893.
    Pubmed CrossRef
  5. Chen, W., H. A. J. Hoitink, A. F. Schmitthenner, and O. H. Tuovinen. 1988. The role of microbial activity in suppression of daming-off caused by Pythium ultimum. Phytopathology 78: 314-322.
    CrossRef
  6. Chen, Y., R. Mei, S. Lu, L. Liu, and J. W. Kloepper. 1994. The use of yield increasing bacteria (YIB) as plant growthpromoting rhizobacteria in chinese agriculture, Utkhede, R. S. and V. K. Gupta (ed), Management of Soil Borne Diseases, Kalyani Publishers, New Delhi, pp. 165-184.
  7. Choi, J. G., Y. S. Kim, W. T. Lee, and S. D. Kim. 1997. Urease gene transfer of antagonistic bacillus subtilis YB-70 and increased antagonistic effect. Kor. J. Microbiol. Biotechnol., 25: 30-36.
  8. Chun, J. S., J. H. Lee, Y. Y. Jung, M. J. Kim, S. Kim, B. K. Kim, and Y. W. Lim. 2007. EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int. J. Syst. Evol. Microbiol. 57: 22592261.
    Pubmed CrossRef
  9. Esitken, A., S. Ercisli, H. Karlidag, and F. Sahin. 2005. Potential use of plant growth promoting rhizobacteria (PGPR) in organic apricot production. Proc. Int. Sci. Conf. Environmentally Friendly Fruit Growing Tartu-Estonia, September 7-9, pp. 90-97.
  10. Imhoff, J. F., and H. G. Truper. 1989. Purple nonsulfur bacteria. pp. 1658-1662. In J. T. Staley et al. (ed.) Bergey’s manual of systematic bacteriology. Vol. 3. Williams and Wilkins, Baltimore.
  11. Ishimaru, C. A. and J. E. Loper. 1992. High-affinity iron uptake systems present in Erwinia carotovora subsp. carotovora include the hydroxamate siderophore aerobactin. J. bacteriol. 174: 2993-3003.
    Pubmed KoreaMed
  12. Jung, H. K., J. R. Kim, S. M. Woo, and S. D. Kim. 2006. An auxin producing plant growth promoting rhizobacterium Bacillus subtilis AH18 which has siderophore-producing biocontol activity. J. Kor. Soc. Appl. Biol. Chem. 7: 94-100.
  13. Jung, H. K., J. R. Kim, S. M. Woo, and S. D. Kim. 2007. Selection of the auxin, siderophore and cellulase- producing PGPR, Bacillus licheniformis K11 and its plant growth promoting mechanisms. J. Kor. Soc. Appl. Biol. Chem. 50:23-28.
  14. Khalid, A., M. Arshad, and Z. A. Zahir. 2004. Screening plant growth-promoting rhizobacteria for improving growth and yield of wheat. J. Appl. Microbiol. 96: 473-480.
    Pubmed CrossRef
  15. Kim, B. S., J. W. A, and K. Y. Cho. 1998. Isolation and identification of Myxobacteria KR025 and searching of their bioactive compounds. Kor. J. Plant Pathol. 14: 345-349.
  16. Kim, J. W., B. K. Jung, C. H. An, J. H. Lim, and S. D. Kim. 2011. Isolation of cellulase-producing plant growth promoting rhizobacteria in pepper farming soil. Yeungnam University.
  17. Kistler, H. C. and U. K. Benny. 1988. Genetic transformation of the fungal plant wilt pathogen, Fusarium oxysporum. Curr. Genet. 13: 145-149.
    CrossRef
  18. Kousik, C. S., J. P. Snow, and R. A. Valverde. 1993. Comparison of double-stranded RNA components and virulence among isolates of Rhizoctonia solani AG-1 IA and AG-1 IB. Phytopathology 84: 44-49.
    CrossRef
  19. Lee, J. M., H. S. Lim, T. H. Chang, and S. D. Kim. 1999. Isolation of siderophore-producing Pseudomonas fluorescens GL7 and its biocontrol activity against root-rot disease. Kor. J. Appl. Microbiol. Biotechnol. 27: 427-432.
  20. Lee, K. E., B. K. Jung, C. H. An, J. H. Lim, and S. D. Kim. 2011. Isolation and identification of siderophore-producing bacteria that has antifungal activity. Yeungnam University.
  21. Gutierrez-Manero, F. J., B. Ramos-Solano, A. Probanza, J. Mehouachi, F. R. Tadeo, and M. Talon. 2001. The plant growth promoting rhizobacteria Bacillus pumilus and Bacillus licheniformis produce high amounts of physiologically active gibberellins. Physiol. Plant. 111: 206-211.
    CrossRef
  22. Loon, L. C. van. 2007. Plant responses to plant growthpromoting rhizobacteria. Eur. J. Plant Pathol. 119: 243-254.
    CrossRef
  23. Lumsden, R. D. 1981. In The Fungal Community: Ecology of mycoparasitism. D. T. Carroll (ed.), Marcel Dekker Inc, N. Y. pp. 295-328.
  24. Moon, S. W., M. Matsuyama, G. M. Go, Y. D. Lee, and Y. B. Go. 1997. N2 fixation and H2 production by a phototrophic bacterium, Chromatium sp. Kor. J. Aquaculture 10: 69-76.
  25. Shon, H. J., B. K. Jung, Y. H. Kim, J. H. Lim, and S. D. Kim. 2011. Isolation and identification of auxin and gibberellin producing photosynthetic bacteria in pepper farming. Yeungnam University.
  26. Tamura, K., J. Dudley, M. Nei, and Sudhir Kumar. 2007. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) Software Version 4.0. Mol. Biol. Evol. 24: 1596-1599.
    Pubmed CrossRef
  27. Teather, R. and P. J. Wood. 1982. Use of congo red-polysaccharide interations in enumeration and characterization of cellulolytic bacteria from the bovine rumen. Appl. Environ. Microbiol. 43: 777-780.
    Pubmed KoreaMed
  28. Thomas, O., G. D. Dieter, and H. Dieter. 1991. IAA synthesis in the biocontrol strain CHO of Pseudomonas fluorescens:role of tryptophan side chain oxidase. J. Gen. Microbiol. 137: 2273-2279.
    Pubmed CrossRef
  29. Xu, J., X. Zhao, X. Han, and Y. Du. 2007. Antifungal activity of oligochitosan against Phytophthora capsici and other plant pathogenic fungi in vitro. Pesticide Biochem. Physiol. 87: 220-228.
    CrossRef

Article Tools

Starts of Metrics

Share this article on :

Related articles in MBL

Most Searched Keywords ?

What is Most Searched Keywords?

  • It is most registrated keyword in articles at this journal during for 2 years.