Contact us
닫기
Article Search
닫기

Microbiology and Biotechnology Letters

View PDF

Microbiol. Biotechnol. Lett. 2010; 38(4): 448-454

DGGE를 이용한 PCE 및 TCE의 혐기적 탈염소화 군집의 미생물 군집분석

Analysis of Microbial Community during the Anaerobic Dechlorination of PCE/TCE by DGGE

Byung-Hyuk Kim , Dae-Hyun Cho , Youl-Boong Sung , Chi-Youg Ahn , Byung-Dae Yoon , Sung-Cheol Koh , Hee-Mock Oh and Hee-Sik Kim

Environmental Biotechnology Research Center, KRIBB, 1Environmental Biotechnology Research Center, KRIBB, 2Env. & Energy Research Center, RIST, 3Environmental Biotechnology Research Center, KRIBB, 4Bioindustrial Process Center, Jeonbuk Branch Institute, KRIBB, 5Department of Environmental Engineering, Korea Maritime University, 6Environmental Biotechnology Research Center, KRIBB, 7Environmental Biotechnology Research Center, KRIBB

Abstract

Go to

This study investigated the effect of PCE and TCE as electron acceptors on the bacterial composition of dechlorinating communities. The enrichment cultures reductively dechlorinating PCE and TCE were developed from three environment samples using acetate as electron donor. The cultures were prepared by sequential enrichment, which was seeded with sediment and dredged soil. Denatured gradient gel electrophresis (DGGE) of 16S rRNA gene fragment was used to compare the microbial communities of these three enrichment cultures. After incubation for 4 weeks, the removal efficiencies of PCE and TCE were highest from Yeocheon site (87.37% and 84.46%, respectively). PCE and TCE as electron acceptors affected the bacterial diversity and community profiles in the enrichment cultures. DGGE analysis showed that the dominant bacteria in PCE and TCE enrichment were belonged to Clostridium sp., Desulfotomaculum sp., and uncultured bacteria.

Keywords: Bioremediation, DGGE, microbial community, TCE, PCE

Article Tools

Starts of Metrics

Share this article on :

Related articles in MBL

Most Searched Keywords ?

What is Most Searched Keywords?

  • It is most registrated keyword in articles at this journal during for 2 years.