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Microbiology and Biotechnology Letters

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Microbiol. Biotechnol. Lett. 2009; 37(4): 340-349

메주로부터 분리한 항진균 및 항세균 활성의 Bacillus polyfermenticus CJ9

Bacillus polyfermenticus CJ9, Isolated from Meju, Showing Antifungal and Antibacterial Activities

Ji-Hye Jung and Hae-Choon Chang

Department of Food and Nutrition, Chosun University, 1Department of Food and Nutrition, Chosun University

Abstract

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A CJ9 bacterial strain, which showed antifungal and antibacterial activities, was isolated from meju and identified as Bacillus polyfermenticus based on Gram staining, biochemical properties, as well as its 16S rRNA sequence. B. polyfermenticus CJ9 showed the antimicrobial activity against the various pathogenic molds, yeasts, and bacteria. The antibacterial activity was stable in the pH 5.0~9.0, but the activity was lost at $37^{circ}C$ for 24 hr. The antifungal activity was stable in the pH range of 3.0~9.0 and reduced at $121^{circ}C$ for 15 min, but antifungal activity was not completely destroyed. The antibacterial activity was completely inactivated by proteinase K, protease, trypsin, and $alpha$-chymotrypsin. The antifungal activity was also completely inactivated by protease and $alpha$-chymotrypsin, and reduced its activity by proteinase which indicated that the antifungal and antibacterial compounds have proteineous nature. The apparent molecular mass of the partially purified antifungal compound, as indicated by using the direct detection method in Tricine-SDS-PAGE, was approximately 1.4 kDa. The molecular mass of the antibacterial compound could not be determined because of its heat-liable characteristic.

Keywords: Bacillus polyfermenticus, antifungal activity, antibacterial activity, proteineous nature

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