Construction of A Bacteriocidal Yeast Producing Bacteriocin OR-7

Ok-Hee  Lee; Min-Kyung  Jang; Dong-Geun  Lee; Jae-Hwa  Lee; Jong-Myung  Ha; Bae-Jin  Ha; Ik-Yong  Ahn; Dong-In  Cho  and Sang-Hyeon  Lee

Microbiol. Biotechnol. Lett. 2008; 36(2): 101-105

박테리오신 OR-7을 생산하는 항균 효모의 제작

Construction of A Bacteriocidal Yeast Producing Bacteriocin OR-7

Ok-Hee Lee , Min-Kyung Jang , Dong-Geun Lee , Jae-Hwa Lee , Jong-Myung Ha , Bae-Jin Ha , Ik-Yong Ahn , Dong-In Cho and Sang-Hyeon Lee

Department of Bioscience and Biotechnology, Silla University, ¹Department of Bioscience and Biotechnology, Silla University, ²Department of Bioscience and Biotechnology, Silla University, ³Department of Bioscience and Biotechnology, Silla University, ⁴Department of Bioscience and Biotechnology, Silla University, ⁵Department of Bioscience and Biotechnology, Silla University, ⁶Youngnam Flour Mills Co., LTD., ⁷Youngnam Flour Mills Co., LTD., ⁸Department of Bioscience and Biotechnology, Silla University

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Abstract

In order to obtain yeast cells producing a bacteriocin OR-7, the 180 bp polynucleotide corresponding to the OR-7 gene including codons for start and stop was chemically synthesized and cloned into pAUR123, an yeast expression vector. Transformed yeast cells exhibited growth inhibition of Bacillus subtilis, Campylobacter jeuni, Escherichia coli and Pseudomonas aeruginosa. This result indicates that yeast cells producing OR-7 possess bacteriocidal properties against both Gram positive B. subtilis and Gram negative C. jejuni, E. coli and P. aeruginosa cells. The recombinant yeast strain constructed in this study can be applied in the food preservative or animal feed.

Keywords: Bacteriocin, Bacillus subtilis, Campylobacter jejuni, Escherichia coli, OR-7, Pseudomonas aeruginosa, Saccharomyces cerevisiae, yeast

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eISSN 2234-7305
pISSN 1598-642X

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Lee OH, Jang MK, Lee DG, Lee JH, Ha JM, Ha BJ, Ahn IY, Cho DI, Lee SH. Construction of A Bacteriocidal Yeast Producing Bacteriocin OR-7. Kor. J. Microbiol. Biotechnol 2008;36:101-105.

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eISSN 2234-7305
pISSN 1598-642X

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박테리오신 OR-7을 생산하는 항균 효모의 제작