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Microbiology and Biotechnology Letters

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Microbiol. Biotechnol. Lett. 2008; 36(2): 101-105

박테리오신 OR-7을 생산하는 항균 효모의 제작

Construction of A Bacteriocidal Yeast Producing Bacteriocin OR-7

Ok-Hee Lee , Min-Kyung Jang , Dong-Geun Lee , Jae-Hwa Lee , Jong-Myung Ha , Bae-Jin Ha , Ik-Yong Ahn , Dong-In Cho and Sang-Hyeon Lee

Department of Bioscience and Biotechnology, Silla University, 1Department of Bioscience and Biotechnology, Silla University, 2Department of Bioscience and Biotechnology, Silla University, 3Department of Bioscience and Biotechnology, Silla University, 4Department of Bioscience and Biotechnology, Silla University, 5Department of Bioscience and Biotechnology, Silla University, 6Youngnam Flour Mills Co., LTD., 7Youngnam Flour Mills Co., LTD., 8Department of Bioscience and Biotechnology, Silla University

Abstract

In order to obtain yeast cells producing a bacteriocin OR-7, the 180 bp polynucleotide corresponding to the OR-7 gene including codons for start and stop was chemically synthesized and cloned into pAUR123, an yeast expression vector. Transformed yeast cells exhibited growth inhibition of Bacillus subtilis, Campylobacter jeuni, Escherichia coli and Pseudomonas aeruginosa. This result indicates that yeast cells producing OR-7 possess bacteriocidal properties against both Gram positive B. subtilis and Gram negative C. jejuni, E. coli and P. aeruginosa cells. The recombinant yeast strain constructed in this study can be applied in the food preservative or animal feed.

Keywords: Bacteriocin, Bacillus subtilis, Campylobacter jejuni, Escherichia coli, OR-7, Pseudomonas aeruginosa, Saccharomyces cerevisiae, yeast

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