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Microbiology and Biotechnology Letters

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Microbiol. Biotechnol. Lett. 2007; 35(4): 292-297

고광도와 질소 결핍이 Haematococcus pluvialis의 색소 생합성에 미치는 영향

Influence of High Light and Nitrate Deprivation on the Carotenoid Biosynthesis in Haematococcus pluvialis

Ji-Hyun Yun , In-Kyu Kwak and Eon-Seon Jin

Department of Life Science, Hanyang University, 1Department of Life Science, Hanyang University, 2Department of Life Science, Hanyang University

Abstract

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The unicellular green alga, Haematococcus pluvialis used as a biological production system for astaxanthin. It accumulates large amounts of the red ketocarotenoid astaxanthin when exposed to various environmental stress such as active oxygen species and high light intensities. To induce astaxanthin biosynthesis of H. pluvialis, cells were incubated in either nitrate free at $25^{circ}C$ under continuous high light intensity ($1,000;{mu}mol$ photons $m^{-2}s^{-1}$) for 2 days or high light stress only. Expressions of astaxanthin biosynthetic genes such as carotenoid hydroxylase, IPP isomerase and ${beta}$-carotene ketolase were monitored under different culture conditions by using real time RT-PCR. All the subjected genes increased their expression under highlight and N-deprivation condition where a large amount of astaxanthin was accumulated.

Keywords: Haematococcus pluvialis, carotenoids, astaxanthin

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