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Microbiology and Biotechnology Letters

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Microbiol. Biotechnol. Lett. 2007; 35(1): 1-10

한반도 중부지방의 벼 뿌리로부터 내생 세균의 분리와 특성 분석

Isolation and Characterization of Endophytic Bacteria from Rice Root Cultivated in Korea

Soo-Young Park , Sung-Hyun Yang , Soo-Keun Choi , Ji-Hyeon Kim , Jong-Guk Kim and Seung-Hwan Park

Laboratory of Microbial Genomics, Systems Microbiology Research Center, KRIBB, 1Laboratory of Microbial Genomics, Systems Microbiology Research Center, KRIBB, 2Laboratory of Microbial Genomics, Systems Microbiology Research Center, KRIBB, 3Laboratory of Microbial Genomics, Systems Microbiology Research Center, KRIBB, 4Department of Microbiology, College of Nature Science, Kyungpook National University, 5Laboratory of Microbial Genomics, Systems Microbiology Research Center, KRIBB

Abstract

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The 44 endophytic bacterial strains were isolated from surface-sterilized root of rice cultivated in seven different locations of Chungcheong province, Korea. Each isolate was introduced into rice seedlings grown gnotobiotically by inoculating scissor-cut first true leaf with cell suspensions, and the colonization capacity of each isolate in root tissue was analyzed at 7 days after inoculation. Sixteen out of 44 isolates were re-isolated from root successfully with the frequency of $10^{3-5}$ CFU/g tissue. Interestingly, seven out of 16 isolates were identified as Burkholderia species. The identity between inoculated strains and re-isolates was confirmed by genomic finger-printing and 16S rDNA sequence analysis. By a confocal laser scanning microscopic observation it was revealed that KJ001 strain, one of the sixteen isolates tagged with gfp colonized in root tissue especially around xylem. Six out of seven Burkholderia strains obtained in this study showed antagonizing activities against seven different fungal pathogens, contain nifH gene, and five of them enhanced growth of cucumber over 30%. The isolates showed no hypersensitive response on tobacco leaves and no pathogenecity in rice. From these results it was found that the endophytic Burkholderia strains will be useful in agriculture to develop a biocontrol agent or a bio-fertilizer.

Keywords: Rice endophyte, PGPR, Burkholderia

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