Isolation and Characterization of Exopolysaccharide Producing Lactic Acid Bacteria from Kimchi

Uyo-Ju  Kim; Hae-Choon  Chang

Microbiol. Biotechnol. Lett. 2006; 34(3): 196-203

김치로부터 Exopolysaccharide 생성 유산균의 분리 및 특성 규명

Isolation and Characterization of Exopolysaccharide Producing Lactic Acid Bacteria from Kimchi

Uyo-Ju Kim and Hae-Choon Chang

Department of Food and Nutrition, Chosun University, ¹Department of Food and Nutrition, Chosun University

Abstract

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Abstract

Three slime-forming lactic acid bacteria were isolated from Kimchi and shown to produce viscous exopolysaccharides (EPS) in sucrose media. The isolated strains, GJ2, C3 and C11, were identified as Leuconostoc kimchii, Leuconostoc citreum and Leuconostoc mesenteroides, respectively, by examining their metabolic characteristics and determining their 16S rDNA sequences. Leu. kimchii GJ2, Leu. citreum C3 and Leu. mesenteroides C11 exhibited high viability (maintained initial viable cell count of $10^8$ CFU/ml) in 0.05 M sodium phosphate buffer (pH 3.0) for 2 h, in artificial gastric juice for 2 h and in 0.3% oxgall for 24 h. When tested, Leu. kimchii GJ2, in particular, displayed antimicrobial activity against pathogenic microorganisms. Leu. kimchii GJ2, Leu. citreum C3 and Leu. mesenteroides C11 produced 21.49 g/l, 16.46 g/l and 22.98 g/l EPS, respectively, in sucrose (5%) medium. The amount of purified EPS extracted from Leu. kimchii GJ2, Leu. citreum C3 and Leu. mesenteroides C11 was 14.61 g/l, 7.73 g/l and 4.77 g/l, respectively. Although the EPS produced by Leu. kimchii GJ2, Leu. citreum C3 and Leu. mesenteroides C11 differed in viscosity, TLC and HPLC analysis revealed that each contained only one type of monosaccharide, glucose. The average molecular mass of EPS produced by Leu. kimchii GJ2 was 306,606 Da.

Keywords: Lactic acid bacteria, Leuconostoc sp., EPS, kimchi, probiotics

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Kim UJ, Chang HC. Isolation and Characterization of Exopolysaccharide Producing Lactic Acid Bacteria from Kimchi. Microbiol. Biotechnol. Lett 2006;34:196-203.

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김치로부터 Exopolysaccharide 생성 유산균의 분리 및 특성 규명