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Microbiology and Biotechnology Letters

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Microbiol. Biotechnol. Lett. 2004; 32(4): 317-321

반응표면분석법에 의한 미역 포자엽의 항산화물질 추출 조건 최적화

Optimization of Extraction Conditions of Antioxidants from Sporophyll of Undaria pinnatifida by Response Surface Methodology

Mi-Yeong Yu, , Gyeong-Hwan Kim, , Jae-U Lee, , Sang-Gwon Kim, and Ji-Yeong Yang,

Division of Food science & Biotechnolology, Pukyong National University, 1Division of Food science & Biotechnolology, Pukyong National University, 2Department of Food Preparation, Changwon College, 3Chung-Ho Sea Food Co., Ltd, 4Division of Food science & Biotechnolology, Pukyong National University

Abstract

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We selected a solvent for producing antioxidants from sporophyll of Undaria pinnatOptimum extraction condition for antioxidant ability(Y) of sporophyll of U. pinnatifida was investigated using response surface methodology(RSM). A compound central design was used with variables ($X_1$)temperature($^{circ}C$) (3.4; 20; 60; 100; 116.6); ($X_2$) pH(1.8; 3; 6; 9; 10.2) and ($X_3$) treatment time(min) (1.7; 10; 30; 50; 58.3). Antioxidant activities were increased with decreasing pH and increasing temperature. The optimal extraction conditions for antioxidant ability(Y) of sporophyll of U. pinnatifida were found to be temperature $51.55^{circ}C$, pH 4.2 and treatment time 28.2 min. Among the variables tested, pH showed greater significant facin extraction of antioxidants from sporophyll of U. pinnatifida.

Keywords: Sporophyll;Undaria pinnatifida;antioxidant activity;response surface methodology;

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