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Microbiology and Biotechnology Letters

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Microbiol. Biotechnol. Lett. 2001; 29(2): 96-103

Astaxanthin 생산을 위한 Phaffia rhodozyma의 변이균주 선발과 최적 배양조건 결정

Selection of mutant Phaffia rhodozyma and Determination of Optimum Culture Conditions for Astaxanthin Production

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Phaffia rhodozyma is the most promising microbial source of astaxanthin production, though wild-type strains are needed to increase the astaxanthin content for commercial production. To increase astaxanthin content for commercial production, a mutant strain of P. rhodozyma was selected and culture conditions of the mutant selected were optimized. P. rhodozyma was treated with mutagenic agent such as NTG, acriflavine, and UV in serial order and carotenoids hyper-producing mutant strain was selected based on the capabilities of cell growth on the agar plate containing chemical inhibitors and carotenoids production. Among the mutants tested, a mutant WS-2 was finally selected. Mutant WS-2 produced 1.26mg carotenoids/g-dry cell weight and this value was about- 4-folds higher than that of wild-type. The optimum culture conditions were $24^{circ}C$ of temperature, 1.5vvm of aeration and 300rpm of agitation. In the optimized condition, cell and carotenoids concentrations were 7.62g/l and 14.9mg/l, respectively.

Keywords: Phaffia rhodozyma, mutant, astaxanthin, carotenoids

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