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Microbiology and Biotechnology Letters

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Microbiol. Biotechnol. Lett. 1999; 27(5): 370-377

Xylanase를 생산하는 내열성 Bacillus속 균주의 분리와 효소생산 조건

isolation of Xylanase-producing Thermo-tolerant Bacillus sp. and Its Enzyme Production

Abstract

Thermo-tolerant bacterium producing the xylanase was isolated from soil and identified as Bacillus pumilus. This strain, named Bacillus pumilus TX703, was able to grow ad produce xylanase at the culture temperature of 5$0^{circ}C$. The maximum xylanase production was obtained when 1%(w/v) birchwood xylan and 1% (w/v) soytone were used as carbon source and nitrogen source, respectively. The biosynthesis of xylanase was under the catabolite repression induced by glucose in the culture medium, and it was completely inhibited in the presence of 0.2% (w/v) glucose. The maximum activity of xylanase was observed from pH8.0 to 9.0 and from 50 to 6$0^{circ}C$ and the enzyme was highly heat-stable, whose activity remained was over 50% at 8$0^{circ}C$, and was quite stable from pH5.0 to 10.0.

Keywords: xylanase, Bacillus pumilus, enzyme production

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