This study was performed to investigate antimicrobial effects and their actions of the ethanol extracts of 12 spices against Lactobacillus plantarum and Leuconostoc mesenteroides, which are related to Kimchi fermentation. The cardamon, thyme, and cumin ethanol extracts among 12 spices showed antimicrobial activities against Lac. plantarum, not against Leu. mesenteroides through paper disc method. The growth of Lac. plantarum was inhibited in MRS broth containing each extracts of cardamon (>4%), thyme (>4%) and cumin (>1%). SDS-PAGE and transmission electron micrographs showed that the cell walls and membranes were disrupted and the cytoplasmic components were leaked in strains treated with ethanol extracts.
Keywords: Kimchi, Lactobacillus plantarum, Leuconostoc mesenteroides, antimicrobial effect, spice
In-Ung Shin , Se-Young Kwun , Eun-Hee Park , Jae-Hyoung Yi and Myoung-Dong Kim
Microbiol. Biotechnol. Lett. 2019; 47(3): 434-440 https://doi.org/10.4014/mbl.1901.01002김재원, 신영재, 심영섭, 유승구, 윤성식
Microbiol. Biotechnol. Lett. 1998; 26(2): 117-121 https://doi.org/10.4014/mbl.1998.26.2.117이신호, 임용숙
Microbiol. Biotechnol. Lett. 1997; 25(2): 224-228 https://doi.org/10.4014/mbl.1997.25.2.224