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Microbiology and Biotechnology Letters

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Microbiol. Biotechnol. Lett. 1998; 26(4): 358-364

새로운 Bacillus thuringiensis NT0423 균주의 제제화

Formulation of a New Bacillus thuringiensis Strain NT0423.

Abstract

New microbial-control agents were prepared with B. thuringiensis strain NT0423 having unique properties which are different with other B. thuringiensis strains belonging to serotype 7[Kor. J. Appl. Entomol. 32: 426-432.]. Three B. thuringiensis formulations designated as BioBact 10%, 20% and 40%, were made with various combinations of adjuvants. These formulations showed good physical properties in wettability, suspensibility, particle size and adherence. In addition the result of SDS-PAGE analysis indicated that $delta$-endotoxins remain stably in all formulations. Among the tested formulations, two wettable powder formulations, BioBact 20% and 40%, comprising 20% and 40% of B. thuringiensis technical powder showed the effective control against diamondback moth larvae (Plutella xylostella) in laboratory and field tests. Especially, when compared with commercial B. thuringiensis formulations (A and B commercial formulations) in field evaluation, BioBact 20% and 40% formulations showed equal activity up to 80% lethality and a good persistence effect which remain on leaves at least 7 days.

Keywords: biodegradation, mixed hydrocarbon, catechol 1,2-dioxygenase, catechol 2,3-dioxygenase, continuous culture

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