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Microbiology and Biotechnology Letters

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Microbiol. Biotechnol. Lett. 1998; 26(3): 200-205

도축 폐혈액 단백질을 이용한 Probiotics생산에서의 동결건조 조건

Optimization of Freeze-drying Conditions for Probiotics Production with Animal Blood Proteins Added Medium.

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A probiotic-strain of Lactobacillus sp. was cultured in bovine blood plasma-based (BBPB) medium and freeze-dried to prepare a probiotic product as an animal feed additive. The cell mass produced in the medium, $5.2{ imes}10^9$ CFU/ml, was high enough to be commercialized and was 74% of that in MRS medium. The survival rate of tactobacillus sp. against freeze-drying was affected by the conditions for treatment of cultured BBPB broth before freeze-drying such as pH adjustment, volume reduction and freezing rate. It was also found that the blood protein hydrolysate remaining in broth also enhanced the survival rate. Among various protective substances, sucrose showed a high stabilizing effect with 10% (w/v) addition, by which the maximum survival rate (48.3%) and viable cell count ($3.0{ imes}10^{10}$ CFU/g) were obtained.

Keywords: bovine blood plasma proteins, slaughterhouse, probiotics, Lactobacillus sp., freeze-drying

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