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Microbiology and Biotechnology Letters

Research Article(보문)

Environmental Microbiology (EM)  |  Microbial Genomes and Metagenomics

Microbiol. Biotechnol. Lett.

Received: October 20, 2024; Revised: December 26, 2024; Accepted: February 20, 2025

Draft Genome Sequence and Antimicrobial Activity of Amycolatopsis japonica SB7-3 Isolated from Soil in Thailand

Apakorn Songsumanus1, Tuangrat Tunvongvinis2, Nisachon Tedsree3, Wongsakorn Phongsopitanun2, and Somboon Tanasupawat2*

1School of Pharmacy, Eastern Asia University, Pathum Thani 12110, Thailand 2Department of Biochemistry and Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand 3Faculty of Science and Arts, Burapha University, Chanthaburi Campus, Chanthaburi 22170, Thailand

Correspondence to :
Somboon Tanasupawat,         Somboon.T@chula.ac.th

Abstract

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This study explores genome analysis and antimicrobial activity of actinobacterium strain SB7-3, isolated from soil. Based on genome sequence analysis, strain SB7-3 was found to be closely related to Amycolatopsis japonica DSM 44213T. The draft genome of strain SB7-3 was 8463214 bp in 19 contigs with a G+C content of 69.10%. The average nucleotide identity-Blast (ANIb) and average nucleotide identity-MUMmer (ANIm) values of strain SB7-3 and A. japonica DSM 44213T were 96.18% and 96.93%, and the digital DNA-DNA hybridization (dDDH) values were 72.90%, respectively. Genomic analysis of strain SB7-3 indicated that the gene encodes enzymes and gene clusters associated with the biosynthesis of bioactive compounds. This strain demonstrated antimicrobial activity against Kocuria rhizophila ATCC 9341, Bacillus subtilis ATCC 6633, and Staphylococcus aureus ATCC 6538.

Keywords: Actinobacteria, Amycolatopsis japonica, antimicrobial activity, genome sequencing

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References

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