Selected D7 antibody inhibit cancer cell growth

Chi Hun   Song; KYUNG HO  HAN

Research Article(보문)

Molecular and Cellular Microbiology | Microbial Genetics, Physiology and Metabolism

Microbiol. Biotechnol. Lett.

Received: August 9, 2024; Revised: September 20, 2024

Selected D7 antibody inhibit cancer cell growth

Chi Hun Song ¹ and KYUNG HO HAN ^1*

Department of Biological Sciences and Biotechnology, Hannam University

Correspondence to :
KYUNG HO HAN, Department of Biological Sciences and Biotechnology, Hannam University, 1646 Yuseongdaero, Yuseong-gu, Daejeon [34054]
Fax : 82426298770, E-mail : kyungho1@hnu.kr

Abstract

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Abstract

We developed to screen for antibodies that promote cell growth in serum-free conditions using a human antibody library. Fetal bovine serum (FBS) is essential for cell growth and proliferation. However, FBS confronts challenges such as high cost, undefined components, and environmental concerns necessitating alternative approaches. To overcome challenges, the antibody library was engineered to be anchored near the cell surface and delivered to cells using a lentivirus to introduce the antibody genes. When treated cells cultured under serum-free conditions, viable cells were obtained and antibodies were selected by PCR. Although selected D7 antibody did not induce cell growth and proliferation under 0% FBS conditions as initially expected, we unexpectedly discovered that D7 antibody significantly inhibits MCF cancer cells in 10% FBS condition. Overall, while we still need to select antibodies to replace FBS in animal cell culture, our findings suggest that D7 antibody, discovered through this novel method, is a promising candidate for future cancer therapies.

Keywords: antibody, serum, antibody library, cancer