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Microbiology and Biotechnology Letters

Research Article(보문)

Molecular and Cellular Microbiology  |  Microbiome

Microbiol. Biotechnol. Lett.

Received: January 29, 2024; Revised: May 12, 2024

Analysis of gut microbiota composition in obese and normal weight Algerian women by 16S rRNA gene amplicon sequencing

Kahina Gribi 1, 2*, Mohammed Sebaihia 2, Mohammed El Amine Bekara 2, Abla Djebbar 2, Manel Zeraoulia 1 and Nihel Klouche-Khelil 1

1Laboratory of Applied Microbiology in Food, Biomedical and Environment, Department of Biology, Faculty of Nature and Life, Earth and Universe Sciences, University Abou Bekr Belkaid of Tlemcen, Tlemcen 13000, Algeria., 2Laboratory of Molecular Biology, Genomics and Bioinformatics, Department of Biology, Faculty of Nature and Life Sciences, University Hassiba Benbouali of Chlef, Chlef 02000, Algeria.

Correspondence to :
Kahina Gribi, Laboratory of Applied Microbiology in Food, Biomedical and Environment, Department of Biology, Faculty of Nature and Life, Earth and Universe Sciences, University Abou Bekr Belkaid of Tlemcen, Tlemcen [13000]
Tel : +213553690029 , Fax : +213027724402, E-mail : kahina.gribi@univ-tlemcen.dz

Abstract

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The prevalence of obesity is increasing throughout the world, imposing a heavy burden on individuals, society and the economy. The gut microbiota (GM) is recognized as an important contributing factor on the development of obesity. Several studies around the world have reported differences in GM composition between obese and lean individuals. However, the GM composition of either obese (OB) or normal weight (NW) Algerian individuals are lacking. Therefore, we conducted the first study in Algeria to characterize and compare the GM composition in OB and NW adult women. Fecal microbiota of 10 Algerian women, 5 OB, with an average Body Mass Index (BMI) of 41.22 ± 5.05, and 5 NW (average BMI of 20.69 ± 0.75 , from the region of Tlemcen in Algeria, were analyzed by 16S rRNA gene amplicon sequencing on NovaSeq 6000 Illumina platform. Alpha and Beta diversity analysis did not reveal significant differences between the two groups. However, the GM composition of our cohort was significantly different from that of Europeans, Americans and Asians, showing predominance of the phyla Firmicutes and Actinobacteria, whereas the phylum Bacteroidota was underrepresented. Obese individuals had a significant increase in the genera Enterococcus, Blautia, Romboutsia, Catenisphaera and Clostridium sensu stricto 1, and a reduced abundance of the genera Bifidobacterium, Ligilactobacillus, Catenibacterium, Collinsella and Akkermansia. This pilot study revealed a distinctive GM composition in Algerian women, highlighting the need for further larger cohort studies to establish the composition of a typical GM composition in the Algerian population.

Keywords: Obesity, Actinobacteria, Gut microbiota, women, Firmicutes, Algeria

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