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Microbiology and Biotechnology Letters

Research Article(보문)

Microbial Biotechnology  |  Cell Culture and Biomedical Engineering

Microbiol. Biotechnol. Lett.

Received: December 4, 2023; Revised: February 1, 2024

Isolation, identification and use of the bacterial strain Ochrobactrum intermedium PDB - 3 for the degradation of the pesticide chlorpyrifos

Diyorbek Qosimov *, Lyudmila Zaynitdinova , Aziza Mavjudova , Muzaffar Muminov and Oybek Shukurov

Institute of Microbiology of the Academy of Sciences of the Republic of Uzbekistan

Correspondence to :
Diyorbek Qosimov, E-mail : diyor-qosimov91@mail.ru

Abstract

One of the serious modern environmental problems is the pollution of natural objects with pesticides, which are highly toxic. A very small amount of pesticides used reaches its target, and the rest pollutes the atmosphere, ending up in soil and water. Chlorpyrifos is a toxic broad-spectrum organophosphate insecticide. Inhibits acetylcholinesterase (AChE) in the peripheral and central nervous system. In children, small amounts of the pesticide cause neurotoxic damage. The toxic effects of chlorpyrifos and its persistence in the environment require its removal from contaminated sites, so it is important to study the biological diversity of chlorpyrifos-degrading microorganisms. This article provides information on determining the degradation of the pesticide chlorpyrifos based on the bacterial strain PDB-3. This strain was isolated from soil contaminated with various pesticides. It was identified as Ochrobactrum intermedium PDB-3 based on morpho-cultural characteristics, MALDI-TOF MS and 16 S rRNA. Studies were conducted for 30 days in sterile soils containing initial concentrations of chlorpyrifos 50; 75; 100 and 125 mg/kg. To determine the degradation of chlorpyrifos, a liquid culture of the strain was added to the soil at three optical densities: at 0, after 24 and 48 hours (OD = 0.03, 0.2 and 0.32). Using GX-MS, it was determined that chlorpyrifos was converted to 3,5,6-trichloro-2-pyridinol (TCP). It was also found that with increasing optical density, rapid decomposition of the initial concentration of chlorpyrifos was observed. Sterile soil without inoculation of strain PDB-3 was used as a control sample.

Keywords: Ochrobactrum intermedium, Optical density, chlorpyrifos, pesticide, bioremediation, biodegradation

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