Article Search
닫기

Microbiology and Biotechnology Letters

보문(Article)

Food Microbiology  |  Food Borne Pathogens and Food Safety

Microbiol. Biotechnol. Lett.

Received: February 7, 2022; Revised: April 4, 2022

Screening of volatile organic compound-producing yeasts and yeast-like fungi against aflatoxigenic Aspergillus flavus

Rujikan Nasanit 1*, Sopin Jaibangyang 1, Tikamporn Onwibunsiri 2 and Pannida Khunnamwong 3, 4*

1Department of Biotechnology, Faculty of Engineering and Industrial Technology, Silpakorn University, Sanamchandra Palace Campus, Nakhon Pathom 73000, Thailand, 2Department of Food Technology, Faculty of Engineering and Industrial Technology, Silpakorn University, Sanamchandra Palace Campus, Nakhon Pathom 73000, Thailand , 3Department of Microbiology, Faculty of Science, Kasetsart University, Jatujak, Bangkok 10900, Thailand, 4Biodiversity Center Kasetsart University (BDCKU), Bangkok 10900, Thailand

Correspondence to :
Rujikan Nasanit, Department of Biotechnology, Faculty of Engineering and Industrial Technology, Silpakorn University, Sanamchandra Palace Campus, Nakhon Pathom 73000, Thailand [73000]
Tel : +66 34 300 463 ext. 209203, Fax : +66 34 300 463, E-mail : nasanit_r@su.ac.th
Pannida Khunnamwong, Department of Microbiology, Faculty of Science, Kasetsart University, Jatujak, Bangkok 10900, Thailand [10900]
Tel : +66 2 562 5444, Fax : +66 2 579 2081, E-mail : fscipik@ku.ac.th

Abstract

Aflatoxin contamination in rice has been documented in a number of studies, with a high incidence in Asian countries. There has been a growing interest in alternative biocontrol strategies to address this issue. In this study, 147 strains of yeasts and yeast-like fungi were screened for their potential in the production of volatile organic compounds (VOCs) that are active against Aspergillus flavus strains that produce Aflatoxin B1 (AFB1). Of these totals, five strains within four different genera showed greater than 50% growth inhibition of some strains of A. flavus. These were Anthracocystis sp. DMKU-PAL124, Aureobasidium sp. DMKU-PAL120, Aureobasidium sp. DMKU-PAL144, Rhodotorula sp. DMKU-PAL99, and Solicococcus keelungensis DMKU-PAL84. VOCs produced by these microorganisms ranged from 4 to 14 compounds including alcohols, alkenes, aromatics, esters and furans under the study condition. It was discovered that the major VOCs produced by the closely-related Aureobasidium strains were distinct. 2-Phenylethanol was the most abundant compound generated by Aureobasidium sp. DMKU-PAL120, while methyl benzeneacetate was the major compound emitted from Aureobasidium sp. DMKU-PAL144. On the other hand, 2-methyl-1-butanol and 3-methyl-1-butanol were significant compounds produced by the other three genera. These antagonists apparently inhibited A. flavus sporulation and mycelial development. Additionally, the reduction of the AFB1 in the fungal-contaminated rice grains was observed after co-incubation with these VOC-producing strains ranging from 37.7 ± 8.3% to 60.3 ± 3.4%. Our findings suggest that these VOC-producing microorganisms are promising biological control agents that could be used against aflatoxin-producing fungi in rice and other agricultural products.

Keywords: aflatoxin B1, Aspergillus flavus, biological control, volatile organic compound

Starts of Metrics

Share this article on :

  • mail

Related articles in MBL

Most KeyWord ?

What is Most Keyword?

  • It is most registrated keyword in articles at this journal during for 2 years.