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Microbiology and Biotechnology Letters


Food Microbiology  |  Bioactive Compounds or Metabolites: Function and Application

Microbiol. Biotechnol. Lett.

Received: January 18, 2022; Revised: March 28, 2022

Antibiofilm activity of Curcuma zedoaria Rosc rhizome extract against methicillin-resistant and susceptible Staphylococcus aureus

Sompong Tabunhan 1* and Parunkul Tungsukruthai 1

Chulabhorn International College of Medicine, Thammasat University, Pathum-Thani 12120, Thailand.

Correspondence to :
Sompong Tabunhan, Chulabhorn International College of Medicine, Thammasat University, 99 Moo 18 Klong Luang, Rangsit, Pathumthani 12120, Thailand [12120]
Tel : +662-5644440-9 Ext. 4465, Fax : +662-986-9213-9, E-mail :


Methicillin-resistant Staphylococcus aureus (MRSA)and Methicillin-susceptible S. aureus (MSSA) are major causes of hospital and community-acquired infections. The treatment of biofilm-related infections caused by these bacteria is a global healthcare challenge. Therefore, the development of alternative therapeutics for treatment is required. Curcuma zedoaria (CZ) Rosc is white turmeric, and an essential oil extracted from this plant has been reported to possess various antimicrobial activities. In the present study, we evaluated the antibiofilm activities of ethanolic extract of CZ rhizome against MRSA and MSSA. The result showed that CZ extract at the highest sub-minimum inhibitory concentration (sub-MIC), 1/2 MIC (0.312 mg/ml) could significantly reduce biofilm production by up to 80%-90% in both tested strains. Subsequently, we further evaluated the ability of the CZ extract to prevent cell-surface attachment to a 96-well plate and extracellular DNA (eDNA) release in biofilm. These results demonstrated the inhibitory effect of CZ on bacterial attachment and eDNA release in biofilm biomass. It is postulated that CZ extract inhibits biofilm formation by preventing eDNA release and cell-surface attachment. Therefore, CZ extract is a potential candidate for the development of alternative treatments to fight against biofilm-associated MRSA infections.

Keywords: Curcuma zedoaria, Antibiofilm, Methicillin-resistant Staphylococcus aureus, Methicillin-susceptible S. aureus, Extracellular DNA, Attachment

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