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Microbiology and Biotechnology Letters

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Microbial Biotechnology (MB)  |  Protein Structure, Function, and Engineering

Microbiol. Biotechnol. Lett.

Received: January 25, 2022; Revised: March 4, 2022; Accepted: March 11, 2022

Characterization of 5-Enolpyruvylshikimate-3- Phosphate Synthase from Colwellia psychrerythraea

Hak Jun Kim*

Department of Chemistry, Pukyong National University, Busan 48513, Republic of Korea

Correspondence to :
Hak Jun Kim,      kimhj@pknu.ac.kr

Psychrophiles have evolved to produce cold-adapted enzymes to enable survival in low-temperature environments. In this study, the cold adaptation of 5-enolpyruvylshikimate-3-phosphate synthase (CpsEPSPS) from Colwellia psychrerythraea, a model psychrophile, was analyzed. The optimum temperature for the activity of CpsEPSPS was found to be 25℃, with 35% activity remaining at 5℃. However, the unfolding temperature of CpsEPSPS was 54℃. This phenomenon is frequently observed in cold-active enzymes. As is the cases for most cold-active enzymes, the Km values of CpsEPSPS for its substrates were higher than those of Escherichia coli EPSPS. These results indicate that CpsEPSPS is cold-adapted, but not perfectly.

Keywords: Psychrophile, 5-enolpyruvylshikimate-3-phosphate synthase, cold adaptation, cold-active enzymes

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