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Microbiology and Biotechnology Letters

보문(Article)

Microbial Biotechnology  |  Cell Culture and Biomedical Engineering

Microbiol. Biotechnol. Lett.

Received: September 17, 2021; Revised: December 29, 2021

Ethanolic Extract of Pancake Mixture Powder supplemented with Helianthus tuberosus Enhances Antidiabetic Effects via Inhibiting Inflammatory Mediator NO Production

Kyoung-Dong Lee 1, Hyeon-Jin Sun 2, Mina Lee 3, Jiyeon Chun 4, Taisun Shin 5, Kap Seong Choi 4* and Sun-Yup Shim 4*

1Department of Oriental Medicine Materials, College of Oriental Medicine, Dongsin University, Naju 58245, Korea, 2Subtropical Horticulture Research Institute, Jeju National University, Jeju, Korea, 3College of Pharmacy, Sunchon National University, Suncheon-Si 57922, Korea, 4Department of Food Science and Biotechnology, College of Life Science and Natural Resources, Sunchon National University, Suncheon 57922, Korea, 5Division of Food and Nutrition, College of Human Ecology, Chonnam National University, Gwangju 61186, Korea.

Correspondence to :
Kap Seong Choi, Department of Food Science and Biotechnology, College of Life Science and Natural Resources, Sunchon National University, Suncheon 57922, Republic of Korea [57922]
Tel : +82-61-750-3257, Fax : +82-61-750-3250, E-mail : chks@scnu.ac.kr
Sun-Yup Shim, Department of Food Science and Biotechnology, College of Life Science and Natural Resources, Sunchon National University, Suncheon 57922, Republic of Korea [57922]
Tel : +82-61-750-3250, Fax : +82-61-750-3250, E-mail : shimsy@scnu.ac.kr

Abstract

Helianthus tuberosus is perennial plant as Asteraceae family and is shown various physiological activities such as analgesic, antipyretic, anti-inflammatory, anti-fungal, anti-spasmodic, aperient, cholagogue, diuretic, spermatogenic, stomachic, and tonic effects. In this study, we investigated the antidiabetic and anti-inflammatory effects of pancake mixure powder (PM) supplemented with H. tuberosus (PMH) in rat skeletal muscle L6 cells and murine macrophage RAW 264.7 cells, respectively. PM and PMH inhibited in vitro ?-glucosidase activity. Glucose consumption was increased by PM and PMH without cytotoxicity in rat myoblast L6 cells. Western blot analysis revealed that PM and PMH down-regulated glycogen synthase kinase (GSK)-3? activation in L6 cells can be down-regulated by PM and PMH. Insulin resistance is increased by inflammation. PM and PMH inhibited inflammatory mediator, nitric oxide (NO) production without cytotoxicity in LPS-induced RAW 264.7 cells. The anti-diabetic and anti-inflammatory of PMH was more stronger than those of PM. Anti-diabetic and anti-inflammatory effects of PMH would be due to functional characteristics of the supplemented H. tuberosus and the presence of garlic and onion used as ingredients of PM. Taken together, our results that addition of functional materials such as H. tuberosus in product has synergic effects and PMH is potential candidate for treatment of diabetes through inhibiting inflammation.

Keywords: Helianthus tuberosus, a-glucosidase activity, GSK-3b, rat myoblast L6 cells, nitric oxide, murine macrophage RAW 264.7 cells

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