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Microbiology and Biotechnology Letters

보문(Article)

Food Microbiology  |  Probiotics in Nutrition and Health

Microbiol. Biotechnol. Lett.

Received: October 20, 2021; Revised: November 12, 2021

Anti-hemolytic and antimicrobial effects against multidrug-resistant bacteria of Enterococcus faecalis isolated from human breast milk

Eun-Ji Yi 1, Jeong-eun Lee 1, So-Yeon Jo 2, Soo-bin Kim 3, Du-na Yu 4, Moochang Kook 2* and Ae-Jung Kim 5*

1Department of Alternative Medicine, Kyonggi University, 2Department of Food & Nutrition, Baewha Women’s University, 3Department of Food and Nutrition, Chung-Ang University, 4Department of Biotechnology, Kyung Hee University, 5The Graduate School of Alternative Medicine, Kyonggi University

Correspondence to :
Moochang Kook, Department of Food & Nutrition, Baewha Women’s University, Seoul 03039, Republic of Korea [03039]
Tel : +82-2-399-0765, Fax : +82-2-737-6711, E-mail : bmse153@gmail.com
Ae-Jung Kim, The Graduate School of Alternative Medicine, Kyonggi University, Seoul 03752, Republic of Korea [03752]
Tel : +82-2-390-5044, Fax : +82-2-313-4131, E-mail : aj5249@naver.com

Abstract

In this study, the hemolysis of Enterococcus faecalis BMSE-HMP strains isolated from the human breast milk were investigated, and the anti-hemolytic and antimicrobial effects on multidrug-resistant(MDR) bacteria were investigated. The enzyme activity of E. faecalis BMSE-HMP 4 strains was measured, and the activities of esterase and esterase lipase were the highest. In addition, no hemolytic reaction was observed in any of the isolates. Then, the anti-hemolytic activity against MDR strains causing hemolysis was performed. E. faecalis BMSE-HMP002 had the highest anti-hemolytic activity against Staphylococcus aureus CCARM 3855 at 75.71±10.00%. And the anti-hemolytic activity against Escherichia coli DC 2 CCARM 0238 and Pseudomonas aeruginosa CCARM 0223 showed the activity of BMSE-HMP001 was highest at 76.92±2.99% and 87.93±1.93%, respectively. As a result of examining the antimicrobial effects against the MDR bacteria Staphylococcus spp., Escherichia spp., Pseudomonas spp., Salmonella spp., Klebsiella spp. and Enterobacter spp., E. faecalis BMSE-HMP strains showed antimicrobial effects in both Gram-positive and Gram-negative strains. Breastfeeding is delivered enterococci into the intestinal tract of newborns by lactation, and its usefulness is attracting attention as it has been reported that enterococci have a potential effect on neonatal immune development. In this study, E. faecalis BMSE-HMP strains were investigated for the hemolysis and antimicrobial effects of MDR bacteria were reviewed to confirm their potential as useful lactic acid bacteria. It is thought that it is necessary to prove safety through additional studies on the antibiotic resistance and toxicity of the E. faecalis BMSE-HMP strains isolated in this study.

Keywords: human breast milk, anti-hemolytic activity, antimicrobial effect, Enterococcus faecalis

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