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Microbiology and Biotechnology Letters

보문(Article)

Food Microbiology  |  Bioactive Compounds or Metabolites: Function and Application

Microbiol. Biotechnol. Lett.

Received: July 15, 2021; Revised: September 2, 2021

Whitening Effect on Melanoma Cells (B16F10) of Abelmoschus esculentus.

DanHee Yoo 1 and InChul Lee 2*

1College of Fusion and Convergence, Seowon University, 2Department of Bio-Cosmetic Science, Seowon University

Correspondence to :
InChul Lee, Seowon University, Cheongju, Chungcheongbuk-do, Korea [28674]
Tel : 0432998491, Fax : 0432998107, E-mail : 5229418@hanmail.net

Abstract

In this study, the whitening effect of Abelmoschus esculentus extract was investigated to confirm its applicability as a cosmetic material. First, to measure the whitening effect, the enzyme inhibitory activity of Abelmoschus esculentus hot water (AEWE) and 70% ethanol extract (AEEE) of tyrosinase was measured. As a result, at the final concentration of 1000 µg/mL, AEWE showed inhibitory activity of 22.2% and AEEE of 32.8%. To measure the whitening effects at the cellular level, the cell viability of AEWE and AEEE were measured in melanoma cell. The cell viability was measured using the MTT assay method. As a result, in AEWE and AEEE at concentrations of 100 µg/mL or less, the cell survival rate was more than 95%. As a result of measuring the melanin contents in melanoma cell, the melanin content was decreased in a concentration-dependent manner in both AEWE and AEEE. The protein expression inhibitory effects of AEWE and AEEE were measured by western blot at concentrations of 5, 10, 50, and 100 µg/mL, and β-actin was used as a positive control. As a result, AEWE showed an effect of 88.1%, 24.8%, 62.2% and 42.9% of microphthalmia-associated transcription factors (MITF), tyrosinase, tyrosinase-related proteins (TRP)-1 and TRP-2 factors, respectively, at a concentration of 100 µg/mL. For the AEEE, MITF, tyrosinase, TRP-1 and TRP-2 factors showed 65.3%, 58.3%, 66.2% and 65.3% effects, respectively, at a concentration of 100 µg/mL. In conclusion, the whitening effects of AEWE and AEEE were verified, and the applicability as a natural cosmetic material was confirmed.

Keywords: Abelmoschus esculentus, B16F10, melanin, whitening effect

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