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Microbiology and Biotechnology Letters

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Microbial Biotechnology  |  Cell Culture and Biomedical Engineering

Microbiol. Biotechnol. Lett.

Received: March 1, 2021; Revised: March 31, 2021

Detection of AluI endonuclease activity by using double stranded DNA-templated copper nanoclusters

Jisu Yang 1 and Jongback Gang 1*

Gachon University

Correspondence to :
Jongback Gang, Department of Chemistry, Gachon University, Sungnam [1342]
Tel : 031-750-5409, Fax : 031-750-5389, E-mail : jbgang@gachon.ac.kr

Abstract

Restriction endonucleases play an important role in molecular cloning, clinical diagnosis, and pharmacological drug studies. In this study, DNA-templated copper nanoclusters (DNA-CuNCs) were used to detect AluI endonuclease activity, due to their high fluorescence emission and rapid synthesis of DNA-CuNCs under ambient conditions. The results showed that AluI activity was detected in a highly sensitive manner at low concentration of AluI endonuclease by the fluorescence intensity of DNA-CuNCs and its inhibition was monitored in the presence of daidzein under optimal conditions.

Keywords: Fluorescence assay, DNA-CuNCs, AluI endonuclease assay

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