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Microbial Biotechnology | Cell Culture and Biomedical Engineering
Microbiol. Biotechnol. Lett.
Jisu Yang 1 and Jongback Gang 1*
Correspondence to :
Jongback Gang, Department of Chemistry, Gachon University, Sungnam 
Tel : 031-750-5409, Fax : 031-750-5389, E-mail : email@example.com
Restriction endonucleases play an important role in molecular cloning, clinical diagnosis, and pharmacological drug studies. In this study, DNA-templated copper nanoclusters (DNA-CuNCs) were used to detect AluI endonuclease activity, due to their high fluorescence emission and rapid synthesis of DNA-CuNCs under ambient conditions. The results showed that AluI activity was detected in a highly sensitive manner at low concentration of AluI endonuclease by the fluorescence intensity of DNA-CuNCs and its inhibition was monitored in the presence of daidzein under optimal conditions.
Keywords: Fluorescence assay, DNA-CuNCs, AluI endonuclease assay