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Microbiology and Biotechnology Letters

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Environmental Microbiology  |  Microbial Ecology and Diversity

Microbiol. Biotechnol. Lett.

Received: March 11, 2021; Revised: April 13, 2021

Biological control activities of Plant Growth Promoting Rhizobacteria from organic and nonorganic rice fields against Rice Sheath Blight pathogen (Rhizoctonia solani Kühn)

Yuniar Harvianti 1 and Rina Sri Kasiamdari 2*

Universitas Gadjah Mada

Correspondence to :
Rina Sri Kasiamdari, Faculty of Biology, Universitas Gadjah Mada, jl. Teknika Selatan, Sekip Utara, Yogyakarta, Indonesia [55281]
Tel : +62274580839, Fax : +62274580839, E-mail : rkasiamdari@ugm.ac.id

Abstract

Rhizoctonia solani is one of the important pathogen causing sheath blight disease on rice. Sheath blight is one of the most difficult diseases to control. Biological control is one of the solutions to control this disease with the use of rhizobacteria. Plant Growth Promoting Rhizobacteria (PGPR) is a rhizosphere bacterium that can be used to enhance plant growth. The composition of the rhizobacteria in organic and nonorganic soil is affected by the chemical characteristics of the soil that influence plant physiology and root exudation patterns. This research aimed to obtain the spesies of rhizobacteria with the PGPR activity from organic and nonorganic rice fields and their capability in R. solani growth suppression. A total of 23 isolates were screened for PGPR activity. Isolates of ISO6, ISO11, ISO15, ISN2, ISN3, and ISN7 were shown to produce high PGPR activity and were selected for in vitro antagonistic activity against R. solani. The six isolates produced IAA ranging from 43,42-75,23 ppm, possessed phosphorus solublization capability and chitinase-producing activity. High activity of R.solani inhibition in vitro were from isolates of ISO6 (54,88%) and ISN7 (83,33%). ISO6 and ISN7 isolates inhibited lesion growth of R. solani on rice leaves by 89% and 100% (without lesion), respectively after 7 days of incubation. Analysis of 16S rRNA sequence revealed that ISO6 isolate was identified as Citrobacter freundii and ISN7 isolate was Pseudomonas aeruginosa.

Keywords: Citrobacter freundii, Plant Growth Promoting Rhizobacteria, Pseudomonas aeruginosa, Rhizoctonia solani, biocontrol, rice sheath blight

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