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Microbiology and Biotechnology Letters

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Food Microbiology  |  Bioactive Compounds or Metabolites: Function and Application

Microbiol. Biotechnol. Lett.

Received: February 24, 2021; Revised: March 30, 2021

Induction of apoptosis in AGS human gastric cancer cells by Platycarya strogilacea leaf extract

Hyeong-Seon Lee 1*

Department of Biomedical Laboratory Science, Jungwon University, Goesan 28024, South Korea

Correspondence to :
Hyeong-Seon Lee, 97, Munhwa-ro, Jeungpyeong-eup, [27921]
Fax : 01040013428, E-mail : biohslee@jwu.ac.kr

Abstract

The present study investigated the anti-cancer activity of methanol extract from Platycarya strogilacea in AGS human gastric cancer cells. We determined the cell viability effect of P. strogilacea using MTS assay. Apoptosis induction and cell cycle arrest were confirmed by fluorescein isothiocyanate (FITC) and propidium iodide (PI) staining using cellometer K2. The expression levels of mRNA of Bcl-2 family were confirmed by reverse transcription polymerase chain reaction (RT-PCR). The cell viability was decreased in a dose-dependent manner treated with different concentration of P. strogilacea. Total, early, and late apoptotic cells were dramatically increased. And, the cell cycle arrested at sub-G1 phase. The mRNA expressions of Bcl-2 and Bcl-xL were reduced, whereas pro-apoptotic factors Bax and Bak were increased in a dose-dependent manner. These results suggested that P. sterogilacea leaf extract induced the powerful apoptotic activity through intrinsic mitochondria pathway.

Keywords: Platycrya strogilacea, AGS human gastric cancer cells, Bcl-2 family, apoptosis

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