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Microbiology and Biotechnology Letters

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Molecular and Cellular Microbiology / Biomedical Sciences  |  Clinical Microbiology and Biomedical Sciences

Microbiol. Biotechnol. Lett.

Received: October 2, 2018; Accepted: October 29, 2018

Protective efficacy of Salmonella Typhimurium strains expressing recombinant Brucella BLS, OMP19, PRPA or SOD proteins in beagles

Won Kyong Kim 1, Jeong Sang Cho 1, Eun Chae Yu 2, Dong Seob Tark 2 and Jin Hur 1*

1Department of Public Health Science, College of Veterinary Medicine Chonbuk National University Special Campus, Iksan 54596, Republic of Korea, 2Chonbuk National University Korea Zoonosis Research Institute, Iksan, 54896, South Korea.

Abstract

The aim of this study is to investigate the protective efficacy of vaccine candidate against Bruccella abortus in Beagles. The vaccines are composed of Salmonella Typhimurium strains expressing recombinant Brucella lumazine synthase (BLS), Omp19, PrpA or SOD proteins of B. abortus. The Beagles in Group A (n = 3) were subcutaneously (SC) inoculated with approximately 4.0 × 109 colony-forming units (CFU/ml) of the S. Typhimurium delivery strain containing only 1 ml of pJHL65. Beagles in Groups B, C, D and E (n = 3 Beagles per group) were SC immunised with 1 ml of approximately 4.0 × 109 CFU/ml of S. Typhimurium expressing the BLS, Omp19, PrpA and SOD proteins, respectively. Serum IgG, interleukin-4 and interferon-gamma concentrations were significantly higher in Groups B–E than in Group A. The severity of brucellosis in terms of infection index and colonisation of B. abortus in tissues was lower in the immunised groups than in the control group. We conclude that each of the S. Typhimurium delivery strains induces significant antigen-specific immune responses and protection in Beagles against infections by B. abortus strain 544. Further investigations are needed to improve the protective efficacy of the candidate vaccine and explore its practical application in Beagles.

Keywords: Brucella abortus, dogs, attenuated Salmonella Typhimurium, immunisation, S. Typhimurium-based B. abortus vaccine

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