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Microbiology and Biotechnology Letters


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Environmental Microbiology (EM)  |  Microbial Ecology and Diversity

Microbiol. Biotechnol. Lett. 2021; 49(3): 374-383

Received: March 11, 2021; Revised: April 13, 2021; Accepted: April 14, 2021

Biological Control Activities of Plant Growth Promoting Rhizobacteria from Organic and Nonorganic Rice Fields against Rice Sheath Blight Pathogen (Rhizoctonia solani Kühn)

Yuniar Harvianti1 and Rina Sri Kasiamdari1,2*

1Graduate Program, Faculty of Biology, Universitas Gadjah Mada. Jl.Teknika Selatan, Daerah Istimewa Yogyakarta 55281, Java, Indonesia 2Plant Systematics Laboratory, Department of Tropical Biology, Faculty of Biology, Universitas Gadjah Mada. Jl.Teknika Selatan, Daerah Istimewa Yogyakarta 55281, Java, Indonesia

Correspondence to :
Rina Sri Kasiamdari,

Rhizoctonia solani is one of the major pathogens that cause sheath blight disease in rice. Sheath blight is one of the most difficult diseases to control. Biological control (with the use of rhizobacteria) is one of the ways to control this disease. Plant Growth Promoting Rhizobacteria (PGPR) is a rhizosphere bacterium that can be used to enhance plant growth. The composition of the rhizobacteria in organic and nonorganic soil is affected by the chemical characteristics of the soil - which influences plant physiology and root exudation patterns. This study aimed to obtain a species of rhizobacteria which shows PGPR activity, from organic and nonorganic rice fields and test their capability to suppress R. solani growth. Out of 23 isolates screened for PGPR activity, the following isolates showed high PGPR activity and were selected for in vitro antagonistic activity testing against R. solani: ISO6, ISO11, ISO15, ISN2, ISN3, and ISN7, The six isolates produced 43,42−75,23 ppm of IAA, possessed phosphorus solubilization capability, and chitinase-producing activity. ISO6 (54.88%) and ISN7 (83.33%) displayed high inhibition capacities against R. solani, in vitro. ISO6 and ISN7 inhibited the growth of R. solani lesions on rice leaves by 89% and 100% (without lesion), respectively, after 7 days of incubation. Analysis of their 16S rRNA sequences revealed that the ISO6 isolate was Citrobacter freundii and ISN7 isolate was Pseudomonas aeruginosa.

Keywords: Citrobacter freundii, plant growth promoting rhizobacteria, Pseudomonas aeruginosa, Rhizoctonia solani, biocontrol, rice sheath blight

Graphical Abstract

  1. Savary S, Willocquet L, Elazegul FA, Castilla NP, Teng PS. 2000. Rice pest constrain in tropical Asia: Quantification of yield losses due to rice pest in range of production situations. Plant Dis. 84:357-369.
  2. Santosa S, Sutarno S, Purwanto EDI, Sajidan S. 2018. Molecular characterization of plant growth promoting rhizobacteria using 16S rRNA sequences in the organic rice field of Sukorejo Village, Central Java, Indonesia. Biodiversitas 19: 2157-2162.
  3. Bulluck LR III, Ristaino JB. 2002. Effect of synthetic and organic soil fertility amendment on southern blight, soil microbial communities, and yield of processing tomato. Phytopathology 92:181-189.
  4. Wolfe DW. 2001. Tales from the Underground: A Natural History of Subterannean Life. pp. 221. Perseus Publishing, Cambridge, Massachusetts. USA.
  5. Gupta G, Parihar SS, Ahirwar NK, Snehi SK, Singh V. 2015. Plant Growth Promoting Rhizobacteria (PGPR): Current and future prospects for development of sustainable agriculture. J. Microbiol. Biochem. Technol. 7: 96-102.
  6. Van Loon LC, Beker P, Pieterse C. 1998. Systemic resistance induced by rhizobacteria. Ann. Rev. Phytopathol. 36: 453-483.
  7. Compant S, Duffy B, Nowak J, Clement C, Barka EA. 2005. Use of plant growth promoting bacteria for biocontrol of plant diseases:principles, mechanisms of action, and future prospects. Appl. Environ. Microbiol. 71: 4951-4959.
    Pubmed KoreaMed
  8. Kazempour MN. 2004. Biological control of Rhizoctonia solani, the causal agent of rice sheath blight by antagonistic bacteria in greenhouse and field conditions. Plant Pathol. J. 3: 88-96.
  9. Weller DM. 1988. Biological control of soil borne plant pathogens in rhizosphere with bacteria. Ann. Rev. Phytopathol. 26: 379-407.
  10. Dilantha F, Nakkeeran S, Yilan Z. 2006. Biosynthesis of Antibiotics by PGPR and Its Relation in Biocontrol of Plant Diseases. pp. 67-109. PGPR Biocontrol Biofert.
  11. Garcia VG, Onco MP, Susan VR. 2006. Review. Biology and systematics of the form genus Rhizoctonia. Spanish J. Agric. Res. 4:55-79.
  12. Wallwork H. 1996. Cereal Root and Crown Diseases Kondinin Group Perth Australia, pp. 14-16.
  13. Smith JD, Kidwell KK, Evans MA, Cook RJ, dan Smiley RW. 2003. Assesment of spring wheat genotypes for disease reaction to Rhizoctonia solani AG 8 in controlled environment and directseeded field evaluation. Crop Sci. 43: 694-700.
  14. Rao S. 1982. Soil Microorganism and Plant Growth. Science Publisher Inc. USA.
  15. Guo Q, Kamio A, Sharma BS, Sagara Y, Arakawa M, Inagaki K. 2006. Survival and subsequent of rice sclerotial diseases fungi, Rhizoctonia oryzae and Rhizoctonia oryzae-sativae, in paddy fields. Plant Dis. 90: 615-622.
  16. Cindy DCB, Sarde CO, Bert V, Tarnaud E, Cochet N. 2012. A standardized method for the sampling of rhizosphere and rhizoplane soil bacteria associated to a herbaceous root sysem. Ann. Microbiol. 6: 471-476.
  17. Thakuria D, Talukdar NC, Goswami C, Hazarika S, Boro RC, Khan MR. 2004. Characterization and screening of bacteria from rhizosphere of rice grown in acidic soils of Assam. Curr. Sci. 86: 978985.
  18. Premono ME, Moawad AM, Vlek PLG. 1996. Effect of phosphate solubilizing Pseudomonas putida on the growth of maize and its survival in the rhizosphere. Indonesian J. Crop Sci. 11: 13-23.
  19. MeÂndez-Bravo A, Cortazar-Murillo EM, Guevara-Avendaño E, Ceballos-Luna O, RodrõÂguez-Haas B, Kiel-MartõÂnez AL, et al. 2018. Plant growth-promoting rhizobacteria associated with avocado display antagonistic activity against Phytophthora cinnamomi through volatile emissions. PLoS One 13: e0194665.
    Pubmed KoreaMed
  20. Shrestha BK, Karki HS, Groth DE, Jungkhun N, Ham JH. 2016. Biological control activities of rice-associated Bacillus sp. strains against sheath blight and bacterial panicle blight of rice. PLoS One 11: e0146764.
    Pubmed KoreaMed
  21. Kumar KVR, Reddy MS, Kloepper JW, Lawrence KS, Groth DE, Miller ME. 2009. Sheath blight disease of rice (Oryza sativa L.)- An overview. Biosci. Biotechnol. Res. Asia 6: 465-480.
  22. Marchesi JR, Sato T, Weightman AJ, Martin TA, Fry JC, Hiom SJ, et al. 1998. Design and evaluation of useful bacterium-spesific PCR primers that amplify genes coding for bacterial 16S rRNA. Appl. Environ. Microbiol. 64: 795-799.
    Pubmed KoreaMed
  23. Degens BD, Vojvodic-Vakovic M. 1999. A sampling strategy to assess the effect of land use on microbial functional diversity in soils. Aust. J. Soil Res. 37: 593-601.
  24. Ge S, Xu H, Ji M, Jiang Y. 2013. Characteristics of soil organic carbon, total nitrogen, and C/N ratio in Chinese apple orchards. Soil Sci. 3: 213-217.
  25. Bastian F, Bouziri L, Nicolardot B, Ranjard L. 2009. Impact of wheat straw decomposition on successional patterns of soil microbial community structure. Soil Biol. Biochem. 41: 262-275.
  26. Fanin N, Hättenschwiler S, Fromin N. 2014. Litter fingerprint on microbial biomass, activity, and community structure in the underlying soil. Plant Soil. 379: 79-91.
  27. Myrold DD. 1999. Transformations of nitrogen. In: Sylvia DM, Fuhrmann JJ, Hartel PG, Zuberer DS (eds) Principles. Appl. Soil Microbiol. 5: 259-294.
  28. Lupwayi NZ, Harker KN, Dosdall LM, Turkington TK, Blackshaw RE, O'Donovan JT, et al. 2009. Changes in functional structure of soil bacterial communities due to fungicide and insecticide applications in canola. Agr. Ecosyst. Environ. 130: 109-114.
  29. Adriano MDL, Gutiérrez F, Dendooven L, Salvador-Figueroa D. 2012. Influence of compost and liquid bioferment on the chemical and biological characteristics of soil cultivated with banana (Musa spp. L.). J. Soil Sci. Plant Nutr. 12: 33-43.
  30. Shen Z, Zhong S, Wang Y, Wang B, Mei X, Li R, et al. 2013. Induced soil microbial suppression of banana fusarium wilt disease using compost and biofertilizers to improve yield and quality. Eur. J. Soil Biol. 57: 1-8.
  31. Joshi M, Shrivastava R, Sharma AK, Prakash A. 2012. Screening of resistant varieties and antagonistic Fusarium oxysporum for biocontrol of Fusarium Wilt of Chilli. Plant Pathol. Microbiol. 3:134.
  32. Hayat R, Ahmed I, Sheirdil RI. 2012. An overview of plant growth promoting rhizobacteria (PGPR) for sustainable agriculture. In Ashraf M, Öztürk M, Ahmad MSA, Aksoy A (eds.). Crop Prod. Agric. Improvement, 3th Ed. Springer, Dordrecht.
  33. Williams GE, Asher MJC. 1996. Selection of rhizobacteria for the control of Phythium ultimum and Aphanomyces cochiliodes on sugerbeet seedlings. Crop Prot. 15: 479-486.
  34. Bormann C, Baier D, Horr I, Raps C, Berger J, Jung G, et al. 1999. Characterization of a novel, antifungal, chitin-binding protein from Streptomyces tendae Tü901 that interferes with growth polarity. J. Bacteriol. 181: 7421-7429.
    Pubmed KoreaMed
  35. Wamishe YA, Jia Y, Gebremariam T, Kelsey C, Belmar S, Mulaw T. 2015. Development of practical diagnostic methods for monitoring rice bacterial panicle blight disease and evaluation of rice germplasm for resistance. In: R.J. Norman and K.A.K. Moldenhauer (eds.). B.R. Wells Rice Research Studies 2014. University of Arkansas Agricultural Experiment Station Research Series 626: 109117.
  36. Schlaberg R, Simmon KE, Fisher MA. 2012. A systematic approach for discovering novel, clinically relevant bacteria. Emerg. Infect. Dis. 18: 422-430.
    Pubmed KoreaMed
  37. Line M. 1990. Identification of nitrogen-fixing enterobacteria from living Sassafras (Atherosperma moschatum Labill.) trees. Plant Soil 125: 149-152.
  38. Kurniawan A, Prihanto AA, Sari SP, Kurniawan A, Asriani E, Sambah AB. 2018. Biochemical characterization of cellulolitic bacteria from mangroves weathered wood in Muntok Sub district, West Bangka regency. Jurnal Sumberdaya Akuatik Indopasifik 2: 1-15.
  39. Hookoom M, Puchooa D. 2013. Isolation and identification of heavy metals tolerant bacteria from industrial and agricultural areas in Mauritius. Curr. Res. Microbiol. Biotechnol. b: 119-123.
  40. Levinson HS, Mahler I. 1998. Phosphatase activity and lead resistance in Citrobacter freundii and Staphylococcus aureus. FEMS Microbiol. Lett. 161: 135-138.
  41. Mahwish Z, Abbasi MK, Hameed S, Rahim N. 2015. Isolation and identification of indigenous plant growth promoting rhizobacteria from Himalayan region of Kashmir and their effect on improving growth and nutrient contents of maize (Zea mays L.). Front. Microbiol. 6: 207.
    Pubmed KoreaMed
  42. Lavakush, Janardan Y, Verma JP. 2012. Isolation and characterization of effective Plant Growth Promoting Rhizobacteria from rice rhizosphere of Indian soil. Asian J. Biol. Sci. 5: 294-303.
  43. Ahmad F, Ahmad I, Khan MS. 2008. Screening of free-living rhizopheric bacteria for their multiple plant growth promoting activities. Microbiol. Res. 168: 173-181.

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