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Microbiology and Biotechnology Letters

Research Article(보문)

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Food Microbiology (FM)  |  Bioactive Compounds or Metabolites: Function and Application

Microbiol. Biotechnol. Lett. 2024; 52(3): 255-263

https://doi.org/10.48022/mbl.2405.05004

Received: May 10, 2024; Revised: July 16, 2024; Accepted: July 25, 2024

카이란(Brassica oleracea L.) 추출물의 대식세포 내에서 LPS에 의한 염증 반응 억제 효과

Inhibitory Effect of Kailan (Brassica oleracea L.) Extract on LPS-Induced Inflammatory Response in Macrophages

DanHee Yoo1 and In Chul Lee2*

1College of Fusion and Convergence, 2Department of Bio-Cosmetic Science, Seowon University, Cheongju 28674, Republic of Korea

Correspondence to :
InChul Lee,       5229418@hanmail.net

Abstract

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In this study, antioxidant and anti-inflammatory activity was studied to confirm the value of kailan (Brassica oleracea L.) as a natural material for cosmetics. For this study measure the antioxidative activity, total polyphenol content was measured, and DPPH and ABTS scavenging activity assays were conducted. As a result of measuring the total polyphenol content of hot water extract of kailan (KRD) and 70% ethanol extract of kailan (KRE), it was found to contain 124.3 mg TAE/100 g and 144.1 mg TAE/100 g, respectively. As a result of DPPH and ABTS radical scavenging ability, it was confirmed that the efficacy was concentration dependent. After treating the cells with LPS, a stimulant, for 2 hours, an experiment was conducted by treating RAW 264.7 cells with KRD and KRE at concentrations of 10, 50, and 100 μg/ml. The nitric oxide production inhibitory activity of KRD and KRE showed an inhibitory effect of about 30% at a concentration of 100 μg/ml. Cells cultured for 18 hours after stimulant treatment were obtained and used in experiments. The cells obtained in this way were lysed, protein and mRNA were extracted, and the expression of inflammatory mediators’ inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) was confirmed. It was confirmed that the protein mRNA expression of iNOS and COX-2, measured through western blot and reverse transcription-PCR, was inhibited in a concentration-dependent manner. Based on this, it is judged that has the potential to be used as a natural material in cosmetics.

Keywords: Antioxidant, anti-inflammatory, kailan, Brassica oleracea L., natural material

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References

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