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Microbiology and Biotechnology Letters

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Food Microbiology (FM)  |  Probiotics in Nutrition and Health

Microbiol. Biotechnol. Lett. 2024; 52(3): 264-274

https://doi.org/10.48022/mbl.2405.05003

Received: May 10, 2024; Revised: July 16, 2024; Accepted: July 25, 2024

Survivability of Encapsulated Bifidobacterium breve BS2-PB3 during Storage and in Simulated Gastric Juice and Bile Salt

Patricia Celine, Marcelia Sugata*, and Tjie Jan Tan

Department of Biology, Faculty of Science and Technology, Universitas Pelita Harapan, Jl. MH. Thamrin Boulevard, Lippo Village, Tangerang 11580, Banten, Indonesia

Correspondence to :
Marcelia Sugata,        marcelia.sugata@uph.edu

Abstract

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Bifidobacterium breve is commonly administered as probiotics in fermented foods or supplements. Accordingly, a sufficient number of live cells must be consumed to experience the benefits of probiotics. Microencapsulation is a technique used to increase the viability and stability of probiotics when exposed to industrial processes, low/high temperature, storage periods, and gastrointestinal conditions. This study aimed to evaluate the effect of microencapsulation with alginate-chitosan and pectin-chitosan matrices on the viability and stability of B. breve BS2-PB3. Microencapsulation was performed using alginate-chitosan (2%, 0.75%) and pectin-chitosan (3%, 0.75%), followed by freeze drying. The encapsulation efficiency of both matrices was 94%, and the survival rate after freeze drying was 96%. After 8 weeks of storage, B. breve BS2-PB3 showed a stable viability, with more than 96% and 83% survival rates at 4℃ and room temperature, respectively. Both encapsulation matrices maintained bacterial viability up to 55% in simulated gastric juice (pH 2) and up to 66% in 0.3% bile salt solution. The administration of microcapsules to mice lowered the fecal pH and increased the defecation frequency. This study demonstrated that microencapsulation using alginate-chitosan and pectin-chitosan could maintain probiotic viability and stability.

Keywords: Alginate, chitosan, pectin, probiotic, shelf life

Graphical Abstract


References

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