Genome Report(Note)
Genome Report | Genome Report
Microbiol. Biotechnol. Lett. 2024; 52(1): 99-101
https://doi.org/10.48022/mbl.2401.01003
Received: January 16, 2024; Revised: February 12, 2024; Accepted: February 26, 2024
Complete Genome Sequences of Two Quinolone-Resistant Methicillin-Resistant Staphylococcus aureus Strains Isolated from Broilers in Korea
Gi Yong Lee and Soo-Jin Yang*
Department of Veterinary Microbiology, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 08826, Republic of Korea
Correspondence to :
Soo-Jin Yang, soojinjj@snu.ac.kr
Fluoroquinolones have been extensively used in treatment or prevention of serious infections in humans and food-producing animals, especially in poultry, due to their broad-spectrum activity to both Gram-positive and -negative pathogens. However, the use of fluoroquinolones in poultry production selects for bacteria carrying genetic determinants for quinolone resistance. Here, we present the complete genome sequences of two methicillin-resistant Staphylococcus aureus (MRSA) strains displaying quinolone resistance, which were isolated from healthy broilers in Korea.
Keywords: Methicillin-resistant Staphylococcus aureus (MRSA), quinolone resistance, broilers
Fluoroquinolones are classified as the highest priority and critically important antimicrobials in human and veterinary medicine, respectively [1, 2]. However, emergence of quinolone resistance, especially in methicillinresistant
The two quinolone-resistant MRSA strains, CJFA-182 and CKFA-361, were isolated from healthy broilers (oropharyngeal and skin swabs, respectively) in Jeolla and Kyeongsang in Korea, respectively [3]. For genome sequencing analysis, total genomic DNA of the CJFA- 182 and CKFA-361 strains were extracted using the Wizard Genomic DNA Kit (Promega, USA) according to the manufacture’s recommendation. Genome sequence libraries were constructed by the Oxford Nanopore MinION system (Oxford Nanopore Technologies, UK) with the Illumina iSeq (Illumina, USA) sequencing platform for an error correction.
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Table 1 . Genetic characteristics of quinolone-resistant MRSA CJFA-182 and CKFA-361 strains.
Genetic features CJFA-182 CKFA-361 Source Broiler Broiler MLST-SCC mec-agr-spa ST692-SCC mec V-I-t2247ST188-SCC mec IVa-I-t189Antimicrobial resistancea AMP-CEF-CIP-CLI-ERY-PEN-TET AMP-CEF-CIP-CLI-ERY-GEN-PEN-SXT Genome size 2,958,125 bp 2,834,769 bp GC content 32.8% 32.9% CDS 2821 2680 No. of contigs 3 2 Chromosome
Plasmidschr (2,927,067 bp)
pCJFA-182_1 (29,583 bp)
pCJFA-182_2 (1,475 bp)chr (2,799,475 bp)
pCKFA-361 (35,294 bp)No. of RNAs 80 79 Antimicrobial resistance genes (location) mecA (chr)blaZ (chr)ermB (chr)tet (S) (chr)blaZ (pCJFA-182_1)tet (L) (pCJFA-182_1)mecA (chr);aph (2’’)-la (pCKFA-361)blaZ (pCKFA-361)ermB (pCKFA-361)dfrE (pCKFA-361)GenBank accession numbers CP085320 (chr) CP085318 (chr) CP085321 (pCJFA-182_1) CP085319 (pCKFA-361) CP085321 (pCJFA-182_2) aAMP, ampicillin; CEF, cefoxitin; CIP, ciprofloxacin; CLI, clindamycin; ERY, erythromycin; GEN, gentamicin; PEN, penicillin; SXT, sulfamethoxazole- trimethoprim; and TET, tetracycline.
CDS, coding sequence; chr, chromosome.
The complete sequences were annotated using Prokka v.1.14.6 [5] and Rapid Annotation using Subsystem Technology (RAST) v.2.0 [6]. Comparative genomic analysis with previsouly published sequences of ST692- MRSA strain K12S0375 (GenBank Accession No. JYGF000000) [7] and ST188-MRSA strain CUHK_188 (GenBank Accession No. JFFV000000) [8] showed 99.93% and 99.7% average nucleotide identity (ANI) values calculated by OrthoANIu algorithm [9]. Analysis of the presence of antimicrobial resistance genes in the two MRSA strains was carried out using ResFinder v.4.4.2 (https://genepi.food.dtu.dk/resfinder) of Center for Genomic Epidemiology and BLAST search. BLAST and ResFinder anaylsis confirmed double point mutations in
The complete genome sequences of two quinonle-resistant MRSA strains CJFA-182 (CP085320-CP085322) and CKFA-361 (CP085318-CP085319) have been deposited in the GenBank sequence database.
Acknowledgments
This study was supported by funds from Research of Korea Centers for Disease Control and Prevention (Project No. 2017NER54060) and Ministry of Food and Drug Safety (2319MFDS012).
Conflict of Interest
The authors have no financial conflicts of interest to declare.
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