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Microbiol. Biotechnol. Lett. 2023; 51(3): 306-308
Sihyun Park1, GyuDae Lee2, Ikwhan Kim3, Yeongyu Jeong4, and Jae-Ho Shin1,2,3*
1Department of Integrative Biology, 2Department of Applied Biosciences, 3NGS Core Facility, Kyungpook National University, Daegu 41566, Republic of Korea
4Division of Agricultural Environment Research, Gyeongsangbuk-do Agricultural Research and Extension Services, Deagu 41404, Republic of Korea
Correspondence to :
Jae-Ho Shin, email@example.com
This research presents the whole-genome sequence of Enterobacter asburiae strain IK3, which was isolated from the rhizosphere soil of soybean (Glycine max). The genome of the strain is composed of a single chromosome with 4 plasmids, total size of 5,084,040 bp, and the GC content is 55.5%.
Keywords: Enterobacter asburiae, whole genome sequencing, agriculture, genome, rhizosphere
This research presents the whole-genome sequence of
The genome sequence of
Genomic DNA was extracted using the Wizard genomic DNA purification kit, following the guidelines provided by Promega, USA. The DNA's quantity and quality were measured using a Qubit 3.0 fluorometer (Thermo Fisher Scientific, USA) and a Nanopore One Spectrophotometer (Thermo Fisher Scientific), respectively. Whole genome sequencing was performed at NGS Core facility (Kyungpook National University, Daegu, Korea). Sequencing was performed through the following methods. The DNA was not pre-selected before the preparation of the sequencing library, which was made following the directions provided by Oxford Nanopore Technologies (ONT) for the use of the SQK-LSK109 ligation sequencing kit and the NEBNext companion module (New England BioLabs, USA). The library was then sequenced on the ONT MinION platform for 72 h using a FLO-MIN111 flow cell. The Guppy v4.4.1 software generated the FASTQ files, and sequences below Phred quality scores of 7 were removed from further analyses. This entire procedure yielded a total of 539,631,756 bp sequenced for
The complete genome sequence data for
This research was supported by the Korea Institute of Planning and Evaluation for Technology in Food, Agriculture and Forestry (IPET) through the Crop Viruses and Pests Response Industry Technology Development Program, funded by the Ministry of Agriculture, Food and Rural Affairs (MAFRA) (321097-3), and with the support of Korea Basic Science Institute (National research Facilities and Equipment center) grant funded by the Ministry of Education (2021R1A6C101A416).
The authors have no financial conflicts of interest to declare.
Amani Sliti, Min-Ji Kim, GyuDae Lee, Yeong-Jun Park, and Jae-Ho ShinMicrobiol. Biotechnol. Lett. 2023; 51(3): 296-299 https://doi.org/10.48022/mbl.2307.07010
Jeong-Seon Kim, Parthiban Subramanian, Seunghwan Kim, Jun Heo, Bong-Sik Yun, and Yiseul KimMicrobiol. Biotechnol. Lett. 2023; 51(3): 328-331 https://doi.org/10.48022/mbl.2307.07002
YoungJae Jo, Amani Sliti, and Jae-Ho ShinMicrobiol. Biotechnol. Lett. 2023; 51(3): 300-302 https://doi.org/10.48022/mbl.2307.07011