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Microbiol. Biotechnol. Lett. 2023; 51(3): 325-327
Hyeon Kyeong Lee, Heung-Soon Park, Eung-Soo Kim, and Si-Sun Choi*
Department of Biological Sciences and Bioengineering, Inha University, Incheon 22212, Republic of Korea
Correspondence to :
Sisun Choi, firstname.lastname@example.org
This paper presents the complete genome sequence of a novel marine actinomycete, Streptomyces sp. MMBL 11-1. The genome of Streptomyces sp. MMBL 11-1 was obtained through next-generation sequencing using the PacBio Sequel system and Illumina platform provided by Macrogen, Korea. The assembled genome consists of five contigs, with a total length of 8,496,900 bp and a G+C content of 71.6%. The genome harbors multiple biosynthetic gene clusters (BGCs) associated with producing microbial natural products (MNPs). The comprehensive genomic information of this type of strain will serve as a valuable resource for identifying other marine actinomycetes strains.
Keywords: Streptomyces sp. MMBL 11-1, marine actinomycetes, whole-genome sequencing, identification, next-generation sequencing
Marine actinomycetes are prokaryotes with significant economic value because they can produce a wide range of natural products. Several genera of marine actinomycetes have been identified, including
The genomic DNA was extracted from the cultured strains using a Wizard® DNA Purification kit (Promega). Both draft gene sequencing and whole genome sequencing were then performed using the extracted genomic DNA. Whole genome sequencing utilized the PacBio Sequel system with PacBio Sequel Microbial Library Construction, conducted by Macrogen Inc.
The Illumina HiSeq platform with TruSeq Nano DNA (350 bp insert size) library was used for draft gene sequencing, and the sequencing process was conducted by Macrogen Inc. Subsequently, the subreads generated from the PacBio Sequel system were assembled using the Microbial assembly application of SMRTlink 10.1.0.119588, based on the Hierarchical Genome Assembly Process (HGAP) . The default options were employed for the analysis within the Microbial assembly application.
Error correction was performed by quality filtering of the Illumina raw reads. Specifically, reads in which 90% of the bases had a phred score of 30 or higher were selected for the error correction step. The assembly was refined using high-quality Illumina reads using Pilon v1.21 .
Gene prediction and basic annotation were performed using Prokka v1.14.6  with the options, --compliant, --rnammer, and --addgenes. Subsequently, the predicted protein sets underwent further annotation using Inter-ProScan v5.30-69.0  and psiblast v2.4.0 , using the EggNOG DB v4.5 . Circular maps illustrating each contig were generated using Circos v0.69.3 .
Whole genome sequencing yielded five contigs, with a total genome size of 8.5 Mb. Contig 1, the largest contig, spanned 8 Mb and was analyzed using the antiSMASH program (Table 1). The analysis revealed the presence of 36 biosynthetic gene clusters (BGCs) expected to be involved in the production of microbial natural products (MNPs). In addition, contig 2 contains two BGCs. Among these BGCs, the nonribosomal peptides (NRPs), polyketides (PKSs), and NRP-PKS hybrids are particularly noteworthy and hold the potential for producing valuable natural products. The complete genome sequences of of
A phylogenetic tree was constructed based on the 16S-23S ITS region sequences of
The authors appreciate the marine-sponge sample provided by the KOIST. This work was funded by the National Research Foundation of Korea (Project No. NRF-2021R1A2C2012203).
The authors have no financial conflicts of interest to declare.
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