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Microbiology and Biotechnology Letters

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Food Microbiology (FM)  |  Probiotics in Nutrition and Health

Microbiol. Biotechnol. Lett. 2022; 50(1): 63-70

https://doi.org/10.48022/mbl.2201.01004

Received: January 12, 2022; Revised: February 18, 2022; Accepted: February 22, 2022

Characterization of Two Cryptic Plasmids from Levilactobacillus zymae GU240

Huong Giang Le1, Min Jae Kim1, Hye Sung Jeon1, Ji Yeon Yoo1, Yun Ji Kang1, Tae Jin Kim1, and Jeong Hwan Kim1,2*

1Division of Applied Life Sci. (BK21 Four), Graduate School, Gyeongsang National University, Jinju 52828, Republic of Korea 2Institute of Agriculture and Life Sci., Gyeongsang National University, Jinju 52828, Republic of Korea

Correspondence to :
Jeong Hwan Kim, E-mail : jeonghkm@gsnu.ac.kr

Two small cryptic plasmids, pHG1 and pHG2, were isolated from Levilactobacillus zymae (formerly Lactobacillus zymae) GU240 and characterized. pHG1 is 1,814 bp in size with a GC content of 37.4% and contains two open reading frames. orf1 can potentially encode a protein of 101 amino acids (aa) with 99% identity with the copy number control protein of Lacticaseibacillus paracasei. orf2 can potentially encode a protein of 230 aa with 99% identity with a replication protein from multiple species. Six inverted repeats (IR I-VI) and six direct repeats (DR I-VI) were found in pHG1. pHG2 is 2,864 bp in size, with a GC content of 39.6%. pHG2 has two orfs. orf1 might encode a protein with 99% identity with the TrsL transmembrane protein. orf2 might encode a protein with 99% identity with plasmid recombination proteins from lactic acid bacteria. Both pHG1 and pHG2 may be useful as frames for constructing lactic acid bacteria–Escherichia coli shuttle vectors.

Keywords: Levilactobacillus zymae, cryptic plasmid, shuttle vector

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