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Microbiology and Biotechnology Letters

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Food Microbiology (FM)  |  Food Borne Pathogens and Food Safety

Microbiol. Biotechnol. Lett. 2022; 50(1): 51-62

https://doi.org/10.48022/mbl.2111.11007

Received: November 9, 2021; Revised: January 18, 2022; Accepted: February 4, 2022

Potential Probiotic Characteristics and Safety Assessment of Lactobacillus rhamnosus SKG34 Isolated from Sumbawa Mare’s Milk

I Nengah Sujaya1, Gede Ngurah Rsi Suwardana2, Kazuyoshi Gotoh3, I Wayan Sumardika4, Komang Ayu Nocianitri5, Ni Putu Sriwidyani6, I Wayan Gede Artawan Eka Putra1, Masakiyo Sakaguchi7, and Ni Nengah Dwi Fatmawati2*

1School of Public Health, Faculty of Medicine, 2Department of Clinical Microbiology, Faculty of Medicine, Universitas Udayana, Bali 80232, Indonesia 3Department of Bacteriology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Japan 4Department of Pharmacology, Faculty of Medicine, 5School of Food Science and Technology, Faculty of Agricultural Technology, 6Department of Pathology Anatomy, Faculty of Medicine, Universitas Udayana, Bali 80232, Indonesia 7Department of Cell Biology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Japan

Correspondence to :
Ni Nengah Dwi Fatmawati,       nnd.fatmawati@unud.ac.id

Lactobacillus rhamnosus SKG34 (LrSKG34), a potential probiotic strain, was successfully isolated from Sumbawa Mare’s milk. Our previous studies showed that the strain is resistant to gastrointestinal conditions, possesses antioxidant activity, and lowers blood cholesterol levels. Further clarification of the potential probiotic characteristics and safety assessment are necessary. This study aimed to evaluate the adhesion of LrSKG34 to Caco-2 cell monolayers and its effect on mucosal integrity in vitro. We also examined the LrSKG34 safety profile based on antimicrobial susceptibility testing, haemolytic activity determination, Caco-2 cell monolayer translocation evaluation, and in vivo investigation of the effect of LrSKG34 on the physiology, biochemical markers, and histopathological appearance of major organs in an animal model. LrSKG34 attached to Caco-2 cell monolayers and maintained mucosal integrity in vitro. The typical resistance of lactobacilli to ciprofloxacin, gentamicin, vancomycin, trimethoprim-sulfamethoxazole, and metronidazole was confirmed for LrSKG34. No haemolytic activity was observed on blood agar plates, and no LrSKG34 translocation was observed in Caco-2 cell monolayers. Administration of LrSKG34 to Sprague- Dawley rats did not adversely affect body weight. No abnormalities in hematological parameters, serum biochemistry levels, or histopathological structures of major organs were observed in LrSKG34-treated rats. Collectively, the results implicate LrSKG34 as a promising and potentially safe probiotic candidate for further development.

Keywords: Lactobacillus rhamnosus SKG34, adhesion, mucosal integrity, safety in vitro, safety in vivo

Graphical Abstract


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