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Microbiology and Biotechnology Letters


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Food Microbiology (FM)  |  Bioactive Compounds or Metabolites: Function and Application

Microbiol. Biotechnol. Lett. 2022; 50(1): 31-39

Received: October 29, 2021; Revised: December 11, 2021; Accepted: December 12, 2021

Melanin 생성 인자 억제 효과를 통한 Mangifera indica L. Peel의 미백효과 연구

A Study on the Whitening Effect of Mangifera indica L. Peel Extracts through Inhibition of Melanin Synthesis Factorㅍ

Hyo-Min Kim1†, Dan-Hee Yoo2†, and In-Chul Lee3*

1College of Pharmacy, Kyungpook National University, Daegu 41566, Republic of Korea 2College of Fusion and Convergence, 3Department of Bio-Cosmetic Science, Seowon University, Cheongju 28674, Republic of Korea

Correspondence to :
InChul Lee,

The purpose of this study was to investigate the whitening effects of hot water (AMPW) and ethanol (AMPE) extracts of Mangifera indica L. peel. To verify the whitening effects, tyrosinase inhibitory activity was measured. 9.51% inhibitory activity, and 35.98% inhibitory activity at 1,000 μg/ml. The effects of AMPW and AMPE on cell viability were measured using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay in B16-F10 melanoma cells. Greater than 95% cell viability was observed at 100 μg/ml. Thus, subsequent experiments were performed at concentrations less than 100 μg/ml. The whitening effects were confirmed by measuring the protein and mRNA expression levels of microphthalmia-associated transcription factor, tyrosinase, tyrosinase-related protein 1 (TRP-1), and TRP-2, which are factors involved in melanin synthesis. Western blotting and reverse transcription-polymerase chain reaction results confirmed that 100 μg/ml AMPW and AMPE showed superior inhibitory effects than the control treatment (alpha-melanocyte stimulating hormone only). Therefore, Mangifera indica L. peel extract had a whitening effect, and thus, has potential as a natural material for use in cosmetics.

Keywords: Mangifera indica L. peel, whitening effects, B16-F10, melanin

Graphical Abstract

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