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Food Microbiology (FM)  |  Bioactive Compounds or Metabolites: Function and Application

Microbiol. Biotechnol. Lett. 2022; 50(1): 31-39

https://doi.org/10.48022/mbl.2110.10012

Received: October 29, 2021; Revised: December 11, 2021; Accepted: December 12, 2021

Melanin 생성 인자 억제 효과를 통한 Mangifera indica L. Peel의 미백효과 연구

A Study on the Whitening Effect of Mangifera indica L. Peel Extracts through Inhibition of Melanin Synthesis Factorㅍ

Hyo-Min Kim1†, Dan-Hee Yoo2†, and In-Chul Lee3*

1College of Pharmacy, Kyungpook National University, Daegu 41566, Republic of Korea 2College of Fusion and Convergence, 3Department of Bio-Cosmetic Science, Seowon University, Cheongju 28674, Republic of Korea

Correspondence to :
InChul Lee,      5229418@hanmail.net

The purpose of this study was to investigate the whitening effects of hot water (AMPW) and ethanol (AMPE) extracts of Mangifera indica L. peel. To verify the whitening effects, tyrosinase inhibitory activity was measured. 9.51% inhibitory activity, and 35.98% inhibitory activity at 1,000 μg/ml. The effects of AMPW and AMPE on cell viability were measured using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay in B16-F10 melanoma cells. Greater than 95% cell viability was observed at 100 μg/ml. Thus, subsequent experiments were performed at concentrations less than 100 μg/ml. The whitening effects were confirmed by measuring the protein and mRNA expression levels of microphthalmia-associated transcription factor, tyrosinase, tyrosinase-related protein 1 (TRP-1), and TRP-2, which are factors involved in melanin synthesis. Western blotting and reverse transcription-polymerase chain reaction results confirmed that 100 μg/ml AMPW and AMPE showed superior inhibitory effects than the control treatment (alpha-melanocyte stimulating hormone only). Therefore, Mangifera indica L. peel extract had a whitening effect, and thus, has potential as a natural material for use in cosmetics.

Keywords: Mangifera indica L. peel, whitening effects, B16-F10, melanin

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  1. Kim DH, Park TS, Kim SG. 2019.Whitening and anti-wrinkle effect of Spirodela polyrhiza extracts. J. Appl Biol. Chem. 62: 391-398.
  2. Ha MJ. 2017. An evaluation of the physiological activities of Opuntia humi fusa fruit extract as cosmetic ingredients for acne. Konkuk University. Seoul, Korea.
  3. Lee SM. 2020. A Study of the Pharmacological Activity of Extracts from Thymus magnus and Thymus quinquecostatus on Skin. Sungshin Women's University. Seoul, Korea.
  4. Han NK, Park CM, Gwon JC, Joung MS, Choi JW. 2014. Whitening effect of Fagopyrum tataricum extract. J. Soc. Cosmet. Sci. Korea 40: 179-186.
  5. Park TS, Kim DH, Son JH. 2015. Whitening effect of Salvia miltorrhiza bunge water extract in human epidermal melanocyte. J. Appl. Biol. Chem. 58: 333-338.
  6. Zhoh CK, Lee MH, Kim IY, Shin JY. 2016. Skin improvement effect of mask pack with snail fermented extract. J. Korean Oil Chemists'Soc. 33: 30-40.
  7. Ann YH, Choi JS. 2006. The whitening effect by water extracts of Bombyx Mori linne. J. Korean Soc. Fashion Beauty 4: 81-86.
  8. Hida T, Kamiya T, Kawakami A, Ogino J, Sohma H, Uhara H, et al. 2020. Elucidation of melanogenesis cascade for identifying pathophysiology and therapeutic approach of pigmentary disorders and melanoma. Int. J. Mol. Sci. 21: 6129.
    Pubmed KoreaMed
  9. Jo JB, Kim MU, Lee EH, Kim YJ, Cho EB, Kang IK, et al. 2018. Whitening effect of extracts from Matricaria chamomilla L. with B16F10 melanoma cells. J. Appl. Biol. Chem. 61: 267-273.
  10. Gillbro JM, Olsson MJ. 2011. The melanogenesis and mechanisms of skin-lightening agents - existing and new approaches. Int. J. Cosmet. Sci. 33: 210-221.
    Pubmed
  11. Hwang HS, Yoo DS, Shim JH. 2016. Whitening effect of banana leaf extract. J. Soc. Cosmet. Sci. Korea 42: 37-43.
  12. Kim PS. 2011. Whitening effects of Angelica dahurica radix ethanol extract. J. Acad.-Ind. Technol. 12: 4038-4045.
  13. Yoo DH, Joo DH, Lee SY, Lee JY. 2015. Antioxidant effect of Nelumbo nucifera G. leaf extract and inhibition of MITF, TRP-1, TRP-2, and tyrosinase expression in a B16F10 melanoma cell line. J. Life Sci. 25: 1115-1123.
  14. Jin KS, Oh YN, Park JA, Lee JY, Jin SJ, Hyun SK, et al. 2012. Antioxidant, anti-melanogenic, and anti-inflammatory activities of Zanthoxylum schinifolium extract and its solvent fractions. Korean J. Microbiol. Biotechnol. 40: 371-379.
  15. Parvez S, Malik KA, Ah Kang S, Kim HY. 2006. Probiotics and their fermented food products are beneficial for health. J. Appl. Microbiol. 100: 1171-1185.
    Pubmed
  16. Jung DH, Shin JH, Cho YY, Son JE. 2015. Development of a twovariable spatial leaf photosynthetic model of irwin mango grown in greenhouse. Protected Horticulture Plant Factory 24:161-166.
  17. An MR, Keum YS, Lee SK. 2015. Comparative analysis of volatile flavor compounds in Taiwan apple mango and Philippines carabao mango. Korean J. Food Sci. Technol. 47: 191-197.
  18. Naef R, Velluz A, Jaquier A. 2006. The perfume of carabao mangoes (Mangifera indica L.): Identification of uncommon unsaturated fatty acid esters in the SPME of the intact fruit. Eur. Food Res. Technol. 222: 554-558.
  19. Ji ST, Youm JW, Yoo JY. 2018. A feasibility study on the cultivation of tropical fruit in Korea: Focused on nango. J. Korean Acad.-Ind. Cooperation Soc. 19: 252-263.
  20. Han SH, Kim JS, Teruya T, Teruya Y, Moromizato I, Lim CK. 2016. Comparison of the fruit qualities, the free radical scavenging activities and mangiferin content of the mango, cv. irwin cultivated in Jeju and Okinawa. Korean J. Hortic. Sci. Technol. 34: 634643.
  21. Jung ES. 2007. Antioxidant and antibacterial activity of extracts from Mangifera indica. Sunchon National University. Sunchun, Korea.
  22. Sivakumar D, Jiang Y, Yahia EM. 2011. Maintaining mango (Mangifera indica L.) fruit quality during the export chain. Food Res. Int. 44: 1254-1263.
  23. Yagi A, Kanbara T, Morinobu N. 1987. Inhibition of mushroomtyrosinase by aloe extract. Planta Med. 53: 515-517.
    Pubmed
  24. Carmichael J, DeGraff WG, Gazdar AF, Minna JD, Mitchell JB. 1987. Evaluation of a tetrazolium based semiautomated colorimetric assay: assessment of chemosensitivity testing. Cancer Res. 47: 936-942.
  25. Kwak JH, Seo UK, Han YH. 2001. Inhibitory effect of Mugwork extracts on tyrosinase activity. Korean J. Biotechnol. Bioeng. 16:220-223.
  26. Song HY, Sung NY, Jung PM, Kang MS, Park WJ, Byun EH. 2015. Whitening effect of green tea seed shell ethanol extracts. J. Korean Soc. Food Sci. Nutr. 44: 1470-1475.
  27. Kim YE, Yang JW, Lee CH, Kwon EK. 2009. ABTS radical scavenging and anti-tumor effects of Tricholoma matsutake sing. (Pine Mushroom). J. Korean Soc. Food Sci. Nutr. 38: 555-560.
  28. Del Marmol V, Beermann F. 1996. Tyrosinase and related proteins in mammalian pigmentation. FEBS Lett. 381: 165-168.
  29. Kim DS. 2003. Sphingosine-1-phosphate decreases melanin synthesis via sustained ERK activation and subsequent MITF degradation. J. Cell Sci. 116: 1699-1706.
    Pubmed
  30. Joo DH, Yoo DH, Lee JY. 2019. A study on the whitening effect of Erigeron annuus (L.) Pers. ethanol extract on melanoma cell (B16F10). Microbiol. Biotechnol. Lett. 47: 148-157.
  31. Kim IH, Lee JH. 2018. Antibacterial and whitening activities of Coffea arabica ethanol extract. Korean Chem. Eng. Res. 56: 245-251.

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