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Microbiology and Biotechnology Letters

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Food Microbiology (FM)  |  Bioactive Compounds or Metabolites: Function and Application

Microbiol. Biotechnol. Lett. 2022; 50(1): 22-30

https://doi.org/10.48022/mbl.2109.09005

Received: September 7, 2021; Revised: November 24, 2021; Accepted: December 11, 2021

Anti-Inflammatory Effects of Abalone (Haliotis discus hannai) Viscera via Inhibition of ROS Production in LPSStimulated RAW 264.7 Cells

Tai-Sun Shin1†, Kap Seong Choi2†, Jiyeon Chun2, Kang-Hee Kho3, Seon Ah Son1, and Sun-Yup Shim2*

1Division of Food and Nutrition, College of Human Ecology, Chonnam National University, Gwangju 61186, Republic of Korea 2Department of Food Science and Technology, College of Life Sciences and Natural Resources, Sunchon National University, Suncheon 57922, Republic of Korea 3Department of Fisheries Science, College of Fisheries and Ocean Sciences, Chonnam National University, Yeosu 59626, Republic of Korea

Correspondence to :
Sun-Yup Shim,       shimsy@scnu.ac.kr

Haliotis discus hannai called abalone, is the valuable marine mollusks and the by-products of abalone processing are viscera. Brownish abalone male viscera (AMV), which have not been reported as having antiinflammatory effects, was extracted with acetone and fractionated by different six acetone/hexane ratios (0, 10, 20, 30, 40, and 100%) using a silica column via in vitro ABTS and DPPH radical and nitric oxide (NO) production assay-guided fractionation. Among the fractions, the acetone/hexane ratio 40%, A40 exhibited the most potent radical scavenging activities and inhibition of lipopolysaccharide (LPS)-induced NO production without cytotoxicity. A40 inhibited LPS-induced intracellular reactive oxygen species (ROS) production in a dose-dependent manner. Western blot analysis revealed that A40 down-regulated the activation of NF-κB, MAPK (ERK 1/2, p-38, and JNK), and inflammatory enzymes, inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)-2. Moreover, this fraction inhibited the generation of pro-inflammatory cytokines such as interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF)-α. These results suggested that AMV containing A40 with anti-inflammatory and anti-oxidantive effects, is the effective therapeutic and functional material for treating inflammatory disorders.

Keywords: Haliotis discus hannai, abalone male viscera, ROS, NF-&kappa,B, MAPK, proinflammatory cytokines

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  1. Chen L, Deng H, Cui H, Fang J, Zuo Z, Deng J, et al. 2018. Inflammatory response and inflammation-associated diseases in organs. Oncotarget 9: 7204-7218.
    Pubmed KoreaMed
  2. Ferrero LM, Nielsen OH, Andersen PS, Girardin SE. 2007. Chronic inflammation: importance of NOD2 and NALP3 in interleukin-1β generation. Clin. Exp. Immunol. 147: 227-235.
    Pubmed KoreaMed
  3. Zhang Q, Zhu B, Li Y. 2017. Resolution of cancer-promoting inflammation: A new approach for anticancer therapy. Front. Immunol. 8: 71.
  4. Sun LD, Wang F, Dai F, Wang YH, Lin D, Zhou B. 2015. Development and mechanism investigation of a new piperlongumine derivative as a potent anti-inflammatory agent. Biochem. Pharmacol. 95: 156-169.
    Pubmed
  5. Duque AG, Descoteaux A. 2014. Macrophage cytokines: involvement in immunity and infectious diseases. Front. Immunol. 5:491.
    Pubmed KoreaMed
  6. Guha M, Mackman N. 2001. LPS induction of gene expression in human monocytes. Cell. Signal. 13: 85-94.
  7. Bogdan C, Rollinghoff M, Diefenbach A. 2000. Reactive oxygen and nitrogen intermediates in innate and specific immunity. Curr. Opin. Immunol. 12: 64-76.
  8. Torres M, Forman HJ. 2003. Redox signaling and the MAP kinase pathways. Biofactors 17: 287-296.
    Pubmed
  9. Medzhitov R, Horng T. 2009. Transcriptional control of the inflammatory response. Nat. Rev. Immunol. 9: 692-703.
    Pubmed
  10. Cobb MH, Goldsmith EJ. 1995. How MAP kinase are regulated. J. Biol. Chem. 270: 14843-14846.
    Pubmed
  11. Marks-Konczalik J, Chu SC, Moss J. 1998. Cytokine-mediated transcriptional induction of the human inducible nitric oxide synthase gene requires both activator protein 1 and nuclear factor κB-binding sites. J. Biol. Chem. 273: 22201-22208.
    Pubmed
  12. Liu T, Zhang L, Joo D, Sun SC. 2017. NF-κB signaling in inflammation. Signal Transduct. Target Ther. 2: e17023.
    Pubmed KoreaMed
  13. Chen Z, Hagler J, Palombella VJ, Melandri F, Scherer D, Ballard D, et al. 1995. Signal-induced site-specific phosphorylation targets IκBα to the ubiquitinproteasome pathway. Genes Dev. 9: 15861597.
    Pubmed
  14. Schonthaler HB, Guinea-Viniegra J, Wagner EF. 2011. Targeting inflammation by modulating the Jun/AP-1 pathway. Ann. Rheumat. Dis. 70: i109-i112.
    Pubmed
  15. Kresge N, Vacquier VD, Stout CD. 2001. Abalone lysin: the dissolving and evolving sperm protein. Bioessays 23: 95-103.
  16. Zhang RJ, Shi Y, Zheng J, Mao XM, Liu ZY, Chen QX, et al. 2018. Effects of polysaccharides from abalone viscera (Haliotis discus hannai Ino) on MGC 803 cells proliferation. Int. J. Biol. Macromol. 106: 587-595.
    Pubmed
  17. Zhou DA, Ma DD, Zhao J, Wan XL, Tong L, Song S, et al. 2016. Simultaneous recovery of protein and polysaccharide from abalone (Haliotis discus hannai Ino) gonad using enzymatic hydrolysis method. J. Food Process. Preserv. 40: 119-130.
  18. Guo S, Wang J, He C, Wei H, Ma Y, Xiong H. 2020. Preparation and antioxidant activities of polysaccharides obtained from abalone viscera by combination of enzymolysis and multiple separation methods. J. Food Sci. 85: 4260-4270.
    Pubmed
  19. Gong F, Chen MF, Chen J, Li C, Zhou C, Hong P, et al. 2019. Boiled abalone byproduct peptide exhibits anti-tumor activity in HT1080 Cells and HUVECs by suppressing the metastasis and angiogenesis in Vitro. J. Agric. Food Chem. 67: 8855-8867.
    Pubmed
  20. Suleria HAR, Masci PP, Gobe GC, Osborne SA. 2017. Therapeutic potential of abalone and status of bioactive molecules: A comprehensive review. Crit. Rev. Food Sci. Nutr. 57: 1742-1748.
    Pubmed
  21. Proestos C, Lytoudi K, Mavromelanidou O, Zoumpoulakis P, Sinanoglou V. 2013. Antioxidant capacity of selected plant extracts and their essential oils. Antioxidants 2: 11-22.
    Pubmed KoreaMed
  22. Blois MS. 1958. Antioxidant determinations by the use of a stable free radical. Nature 181: 1199-1200.
  23. Raghav SK, Gupta B, Shrivastava A, Das HR. 2007. Inhibition of lipopolysaccharide-inducible nitric oxide synthase and IL-1β through suppression of NF-κB activation by 3-(1'-1'-dimethylallyl)6-hydroxy-7-methoxy-coumarin isolated from Ruta graveolens L. Eur. J. Pharmacol. 560: 69-80.
    Pubmed
  24. Bjelakovic G, Nikolova D, Gluud C. 2014. Antioxidant supplements and mortality. Curr. Opin. Clin. Nutr. Metab. Care 17: 40-44.
  25. Yangthong M, Hutadilok-Towatana N, Phromkunthong W. 2009. Antioxidant ac- tivities of four edible seaweeds from the southern coast of Thailand. Plant Foods Hum. Nutr. 64: 218-223.
    Pubmed
  26. Finkel T. 2011. Signal transduction by reactive oxygen species. J. Cell Biol. 194: 7-15.
    Pubmed KoreaMed
  27. Kaur G, Sharma A, Bhatnagar A. 2021. Role of oxidative stress in pathophysiology of rheumatoid arthritis: insights into NRF2KEAP1 signalling. Autoimmunity 54: 385-397.
    Pubmed
  28. Marin DP, Bolin AP, Campoio TR, Guerra BA, Otton R. 2013. Oxidative stress and antioxidant status response of handball athletes:Implications for sport training monitoring. Int. Immunopharmacol. 17: 462-470.
    Pubmed
  29. Kwon MJ, Kim B, Lee YS, Kim TY. 2012. Role of superoxide dismutase 3 in skin infammation. J. Dermatol. Sci. 67: 81-87.
    Pubmed
  30. Dinarello CA. 2009. Immunological and inflammatory functions of the interleukin-1 family. Annu. Rev. Immunol. 27: 519-550.
    Pubmed
  31. Andus T, Geiger T, Hirano T, Kishimoto T, Heinrich PC. 1988. Action of recombinant human interleukin 6, interleukin 1β and tumor necrosis factor α on the mRNA induction of acute?phase proteins. Eur. J. Immunol. 18: 739-746.
    Pubmed
  32. Baggiolini M, Clark-Lewis I. 1992. Interleukin-8, a chemotactic and inflammatory cytokine. FEBS Lett. 307: 97-101.
  33. Molofsky AB, Savage AK, Locksley RM. 2015. Interleukin-33 in tissue homeostasis, injury, and inflammation. Immunity 42:1005-1019.
    Pubmed KoreaMed
  34. Murakami A, Ohigashi H. 2007. Targeting NOX, INOS and COX-2 in inflammatory cells: chemoprevention using food phytochemicals. Int. J. Cancer 121: 2357-2363.
    Pubmed
  35. Urban MK. 2000. COX-2 specific inhibitors offer improved advantages over traditional NSAIDs. Orthopedics 23: S761-764.
    Pubmed

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