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Microbiology and Biotechnology Letters


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Food Microbiology (FM)  |  Probiotics in Nutrition and Health

Microbiol. Biotechnol. Lett. 2021; 49(4): 519-527

Received: October 20, 2021; Revised: November 12, 2021; Accepted: November 22, 2021

모유에서 분리한 Enterococcus faecalis의 다제내성 균에 대한 항용혈 및 항균 효과

Anti-Hemolytic and Antimicrobial Effects against Multidrug-Resistant Bacteria of Enterococcus faecalis Isolated from Human Breast Milk

Eun-Ji Yi1, Jeong-eun Lee1, So-Yeon Jo2, Soo-bin Kim3, Du-na Yu4, Moochang Kook2*, and Ae Jung Kim5*

1Department of Alternative Medicine, Kyonggi University, Seoul 03746, Republic of Korea 2Department of Food and Nutrition, Baewha Women’s University, Seoul 03039, Republic of Korea 3Department of Food and Nutrition, Chung-Ang University, Anseong 17546, Republic of Korea 4Department of Biotechnology, Kyung Hee University, Yongin 17104, Republic of Korea 5The Graduate School of Alternative Medicine, Kyonggi University, Seoul 03752, Republic of Korea

Correspondence to :
Moochang Kook,
Ae-Jung Kim, 

In this study, the hemolysis of Enterococcus faecalis BMSE-HMP strains, isolated from human breast milk, was investigated, and the anti-hemolytic and antimicrobial effects on multidrug-resistant (MDR) bacteria were investigated. The enzyme activity of E. faecalis BMSE-HMP 4 strains was measured, and it was found that the activities of esterase and esterase lipase were the highest. In addition, no hemolytic reaction was observed in any of the isolates. Subsequently, the anti-hemolytic activity against MDR strains causing hemolysis was evaluated. E. faecalis BMSE-HMP002 had the highest anti-hemolytic activity against Staphylococcus aureus CCARM 3855 at 75.71 ± 10.00%. The anti-hemolytic activity against Escherichia coli DC 2 CCARM 0238 and Pseudomonas aeruginosa CCARM 0223 showed that the activity of BMSE-HMP001 was highest at 76.92 ± 2.99% and 87.93 ± 1.93%, respectively. Examination of the antimicrobial effects against the MDR bacteria Staphylococcus spp., Escherichia spp., Pseudomonas spp., Salmonella spp., Klebsiella spp., Enterobacter spp., and E. faecalis BMSE-HMP strains showed antimicrobial effects against both gram-positive and gram-negative strains. Breastfeeding delivers enterococci into the intestinal tract of newborns by lactation, and its usefulness is attracting attention as it has been reported that enterococci have a potential effect on neonatal immune development. In this study, the hemolytic and antimicrobial effects of E. faecalis BMSE-HMP strains on MDR bacteria were investigated, to confirm their potential as useful lactic acid bacteria. Additional studies on the antibiotic resistance and toxicity of the E. faecalis BMSE-HMP strains, isolated in this study, are necessary to prove it safe for use.

Keywords: Human breast milk, anti-hemolytic activity, antimicrobial effect, Enterococcus faecalis

Graphical Abstract

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