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Microbiology and Biotechnology Letters

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Microbial Biotechnology (MB)  |  Cell Culture and Biomedical Engineering

Microbiol. Biotechnol. Lett. 2021; 49(4): 543-551

https://doi.org/10.48022/mbl.2109.09016

Received: September 29, 2021; Revised: October 18, 2021; Accepted: November 16, 2021

Anti-Inflammatory and Anti-Fibrotic Activities of Nocardiopsis sp. 13G027 in Lipopolysaccharides- Induced RAW 264.7 Macrophages and Transforming Growth Factor Beta-1-Stimulated Nasal Polyp-Derived Fibroblasts

Grace Choi1*, Geum Jin Kim2,3, Hyukjae Choi2,3, Il-Whan Choi4, and Dae-Sung Lee1

1Department of Genetic Resources Research, National Marine Biodiversity Institute of Korea, Chungcheongnam-do 33662, Republic of Korea, 2College of Pharmacy, Yeungnam University, Gyeongsangbuk-do 38531, Republic of Korea, 3Research Institute of Cell Culture, Yeungnam University, Gyeongsangbuk-do 38531, Republic of Korea, 4Department of Microbiology and Immunology, Inje University College of Medicine, Busan 49267, Republic of Korea

Correspondence to :
Grace Choi,      gchoi@mabik.re.kr

Nocardiopsis species produce bioactive compounds, such as antimicrobial and anti-cancer agents and toxins. However, no reports have described their anti-inflammatory and anti-fibrotic effects during nasal polyp (NP) formation. In this study, we investigated whether marine-derived bacterial Nocardiopsis sp. 13G027 exerts anti-inflammatory and anti-fibrotic effects on lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages and transforming growth factor (TGF)-β1-induced NP-derived fibroblasts (NPDFs). Nitric oxide (NO) and prostaglandin E2 (PGE2) levels were analyzed. Extract from Nocardiopsis sp. 13G027 significantly inhibited the upregulation of NO and PGE2 in LPS-activated RAW 264.7 macrophages. The expression of mitogen-activated protein kinases (MAPKs) and protein kinase B (Akt/PKB) in LPS-induced RAW 264.7 macrophages was evaluated; smooth muscle alpha-actin (α-SMA), collagen type I (Col-1), and fibronectin also phosphorylated small mothers against decapentaplegic (SMAD) 2 and 3 in TGF-β1–stimulated NPDFs. The Nocardiopsis sp. 13G027 extract suppressed the phosphorylation of MAPKs and Akt and the DNA-binding activity of activator protein 1 (AP-1). The expression of pro-fibrotic components such as α- SMA, Col-1, fibronectin, and SMAD2/3 was inhibited in TGF-β1–exposed NPDFs. These findings suggest that Nocardiopsis sp. 13G027 has the potential to treat inflammatory disorders, such as NP formation.

Keywords: Nasal polyp, Nocardiopsis sp. 13G027, anti-inflammatory, anti-fibrotic

Graphical Abstract


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