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Microbiology and Biotechnology Letters

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Food Microbiology (FM)  |  Bioactive Compounds or Metabolites: Function and Application

Microbiol. Biotechnol. Lett. 2021; 49(4): 485-492

https://doi.org/10.48022/mbl.2107.07006

Received: July 15, 2021; Revised: September 2, 2021; Accepted: September 9, 2021

B16F10 세포에서의 오크라 추출물의 미백 활성 검증

Whitening Effect of Abelmoschus esculentus on Melanoma Cells (B16F10)

Dan-Hee Yoo1 and In-Chul Lee2*

1College of Fusion and Convergence, Seowon University, Cheongju 28674, Republic of Korea 2Department of Bio-Cosmetic Science, Seowon University, Cheongju 28674, Republic of Korea

Correspondence to :
InChul Lee,      5229418@hanmail.net

In this study, the whitening effect of Abelmoschus esculentus extract was investigated to confirm its applicability in cosmetics. To determine the whitening effect, the tyrosinase-inhibitory activity of Abelmoschus esculentus hot water extract (AEWE) and 70% ethanol extract (AEEE) was measured. At the final concentration of 1000 μg/ml, AEWE showed an inhibitory activity of 22.2% and AEEE of 32.8%. To determine the whitening effect at the cellular level, the viability of melanoma cells treated with AEWE and AEEE was evaluated using the MTT assay. At concentrations of 100 μg/ml or less, both AEWE- and AEEE-treated groups showed cell survival rates of >95%. Furthermore, in both AEWE- and AEEE-treated melanoma cells, the melanin content decreased in a concentration-dependent manner. The inhibitory effects of AEWE and AEEE used at 5, 10, 50, and 100 μg/ml on protein expression were measured by western blot, with β-actin as the positive control. At a concentration of 100 μg/ml, AEWE showed an inhibitory effect of 88.1%, 24.8%, 62.2%, and 42.9% on microphthalmia-associated transcription factors (MITF), tyrosinase, tyrosinase-related proteins (TRP)- 1, and TRP-2 factors, respectively. At the same concentration, AEEE showed inhibitory effect of 65.3%, 58.3%, 66.2%, and 65.3% against MITF, tyrosinase, TRP-1, and TRP-2 factors, respectively. In conclusion, the whitening effects of AEWE and AEEE were verified, and their applicability as a natural ingredient in cosmetics was confirmed.

Keywords: Abelmoschus esculentus, B16F10, melanin, whitening effect

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