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Microbiology and Biotechnology Letters

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Molecular and Cellular Microbiology (MCM)  |  Functional Genomics and Systems Biology

Microbiol. Biotechnol. Lett. 2021; 49(3): 440-448

https://doi.org/10.48022/mbl.2107.07001

Received: July 2, 2021; Revised: August 23, 2021; Accepted: August 24, 2021

Comparative Transcriptome Analysis of Caryophyllene- Treated Helicobacter pylori

Hyun Jun Woo1†, Ji Yeong Yang2†, Hye Jin Kwon3, Hyun Woo Kim3, Sa-Hyun Kim1*, and Jong-Bae Kim3*

1Department of Clinical Laboratory Science, Semyung University, Jaecheon 27136, Republic of Korea, 2Division of Crop Foundation, National Institute of Crop Science (NICS), Rural Development Administration (RDA), Wanju, 55365, Republic of Korea, 3Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University, Wonju 26493, Republic of Korea

Correspondence to :
Sa-Hyun Kim,       science4us@semyung.ac.kr
Jong-Bae Kim,      kimjb70@yonsei.ac.kr

Helicobacter pylori (H. pylori) establishes long-term infection which is certainly associated with severe gastric diseases such as peptic ulceration and gastric cancer. The expression level of pathogenic genes could be regulated when H. pylori is exposed to the antibacterial agent. In this study, we analyzed the transcriptional changes of H. pylori genes induced by β-caryophyllene. We used next-generation sequencing (NGS) to analyze the RNA expression changes, then reverse transcription-polymerase chain reaction (RT-PCR) was performed as required to verify the results. The NGS result showed that a total of 30 out of 1,632 genes were differentially expressed by β-caryophyllene treatment. Eleven genes associated with DNA replication, virulence factors and T4SS components were significantly down-regulated. RT-PCR confirmed that treatment reduced the expression level of 11 genes. RT-PCR equally showed the reduced expression of 11 genes (dnaE, dnaN, holB, gyrA, cagA, vacA, secA, flgE, virB2, virB4 and virB8) by β-caryophyllene treatment. These results suggest that β-caryophyllene can modulate a variety of H. pylori pathogenic determinants and can be a potential therapeutic agent for H. pylori infection.

Keywords: Helicobacter pylori, caryophyllene, next-generation sequencing, RNA-sequencing

Graphical Abstract


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