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Environmental Microbiology (EM)  |  Microbial Ecology and Diversity

Microbiol. Biotechnol. Lett. 2021; 49(3): 356-366

https://doi.org/10.48022/mbl.2106.06007

Received: June 11, 2021; Revised: August 19, 2021; Accepted: August 24, 2021

Biosurfactant Production by Marine Actinomycetes Isolates Streptomyces althioticus RG3 and Streptomyces californicus RG8 as Promising Sources of Antimicrobial and Antifouling Effects

Moaz M. Hamed1*, Mohamed A.A. Abdrabo1, and Asmaa M. Youssif2

1Marine Microbiology Laboratory, Marine Environmental Division, National Institute of Oceanography and Fisheries, Egypt (NIOF) 2Department of Botany and Microbiology, Faculty of Science, Alexandria University, Egypt

Correspondence to :
Moaz Hamed,  Moaz-micro@hotmail.com

Two marine actinobacterial isolates, RG3 and RG8, were identified using 16Sr DNA as Streptomyces althioticus RG3 and Streptomyces californicus RG8 and submitted to the database of genetic information with accession numbers MW661230 and MW661234, respectively; they were found to have emulsification indexes of 60 ± 2.5% and 53 ± 2.2%, respectively. The biosurfactants obtained were stable at a temperature of 35℃ for both strains; they were stable at 10% NaCl, in the case of S. althioticus RG3 and at 10–15% NaCl in the case of Str. californicus RG8; both strains produced the most biosurfactant when exposed to alkaline conditions. We characterized the biosurfactants, including features such as their chemical composition, using Fourier transform infrared spectroscopy analysis. The antimicrobial activity of the biosurfactant extracts was evaluated using the well diffusion method against Vibrio alginolyticus MK170250, Escherichia coli ATCC 8739, Pseudomonas aeruginosa ATCC 4027, and Staphylococcus aureus ATCC 25923. S. althioticus RG3 biosurfactants were found to have better antimicrobial activity than those of Str. californicus RG8, indicating that they may be used in pharmaceutical industries and in the manufacture of antifouling products.

Keywords: Biosurfactants, actinomycetes, antimicrobial, red Sea, Egypt

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