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Microbiology and Biotechnology Letters

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Fermentation Microbiology (FM)  |  Strain Isolation and Improvement

Microbiol. Biotechnol. Lett. 2021; 49(4): 552-558

https://doi.org/10.48022/mbl.2106.06004

Received: June 7, 2021; Revised: July 23, 2021; Accepted: August 9, 2021

한국전통발효식품에서 분리한 Probiotics의 특징 및 Synbiotics 항균활성 효과

Characteristics of Probiotics Isolated from Korean Traditional Foods and Antibacterial Activity of Synbiotics

Chae-Yun Moon and Moon-Soo Heo*

Department of Aquatic Biomedical Sciences, Jeju National University, Jeju 63243, Republic of Korea

Correspondence to :
MoonSoo Heo,      msheo@jejunu.ac.kr

Traditional foods are manufactured according to the characteristics of each region for the nations of the world. Korea has mainly farmed, and seasonings have developed around rice and vegetables. In fermented foods, lactic acid bacteria such as Lactobacillus sp. and Pediococcus sp. and Bacillus sp. were isolated and identified from fermented foods. In this study, lactic acid bacteria were isolated and identified from commercially available traditional Korean fermented foods, and candidate strains were selected through antibacterial activity tests on human and fish disease bacteria. Thereafter, the final strain was selected by examining the resistance to simulated gastric and intestinal fluids, and hemolysis. The three (M1, K1, C13) final selected latic acid bacteria were miced with prebiotics and the antibacterial activity of synbiotics was evaluated. As for the fist antibacterial activity result, C13 showed high antibacterial acitivity in human diseases and fish diseases. Then, M1, K1 and C13, which did not produce β-haemolysis and were resistant to simulated gastric and intestinal fluids, were subjected to the second antibacterial activity of synbiotics. When the three prebiotics (FOS, GOS, Inulin) and probiotics (M1, K1, C13) were mixed, the antibacterial activity was increased or inhibited. Based on the 16S rRNA gene sequencing results, K1 and M1 were analyzed as Bacillus tequiensis 99.72%, Bacillus subtilis 99.65%, Bacillus inaquosorum 99.72%, Bacillus cabrialesii 99.72%, Bacillus stercoris 99.58%, Bacillus spizizenii 99.58%, Bacillus halotolerans 99.58%, and Bacillus mojavensis 99.51%. And C13 was analyzed as Bacillus velezensis 99.71%, Bacillus nematocida 99.36%, Bacillus amyloliquefaciens 99.44%, Bacillus atrophaeus 99.22%, and Bacillus nakamurai 99.44%.

Keywords: Synbiotics, probiotics, prebiotics, phylogenetic analysis, 16S rDNA, antibacterial activity

Graphical Abstract


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